Pmch (BC048534) Mouse Tagged ORF Clone

CAT#: MG201335

Pmch (GFP-tagged) - Mouse pro-melanin-concentrating hormone (cDNA clone MGC:58461 IMAGE:6533154)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pmch
• Synonyms: MCH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201335 representing BC048534
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAGATGACTCTCTCTTCCTACATGTTAATGCTGGCTTTTTCTTTGTTTTCTCAAGGTATTTTAC
  TTTCAGCTTCCAAGTCCATAAGGAATTTGGAAGATGACATAGTATTTAATACATTTCGAATGGGGAAAGC
  CTTTCAGAAGGAAGATACTGCAGAAAGATCCGTTGTCGCCCCTTCTCTGGAACAATACAAAAACGACGAG
  AGCGGTTTCATGAACGATGATGACAATAAGAATTCAAAGAACACAGGCTCCAAACAGAATCTTGTAACTC
  ACGGGCTGCCACTGAGTCTGGCTGTAAAACCTTACCTCGCTCTGAAAGGATCCGTAGCCTTCCCAGCTGA
  GAATGGAGTTCAGAATGCTGAGTCCACACAGGAAAAGAGAGAAACTGGGGATGAAGAAAACTCAGCTAAA
  TTTCCCATAGGAAGGAGAGATTTTGACATGCTCAGGTGTATGCTGGGAAGAGTCTACCGACCATGTTGGC
  AAGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201335 representing BC048534
  Red=Cloning site Green=Tags(s)
  
  MAKMTLSSYMLMLAFSLFSQGILLSASKSIRNLEDDIVFNTFRMGKAFQKEDTAERSVVAPSLEQYKNDE
  SGFMNDDDNKNSKNTGSKQNLVTHGLPLSLAVKPYLALKGSVAFPAENGVQNAESTQEKRETGDEENSAK
  FPIGRRDFDMLRCMLGRVYRPCWQV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC048534
• ORF Size: 497 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC048534, AAH48534
• RefSeq Size: 774
• RefSeq ORF: 497
• Locus ID: 110312
• Gene Summary: This gene encodes a preproprotein that is cleaved to produce three mature peptides: melanin concentrating hormone, neuropeptide-glutamic acid-isoleucine (NEI), and neuropeptide-glycine-glutamic acid (NGE). The proprotein is processed differently depending on the tissue where it is expressed. Melanin concentrating hormone is involved in the regulation of food intake, energy homeostasis, and sleep-wake behavior. Disruption of this gene is associated with resistance to diet-induced obesity. [provided by RefSeq, May 2013]