Atp6v0b (NM_033617) Mouse Tagged ORF Clone

CAT#: MG202139

Atp6v0b (GFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit B (Atp6v0b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atp6v0b
• Synonyms: 2310024H13Rik; Atp6f; VMA16
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202139 representing NM_033617
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGGGCTGGAGTTGCTCTACCTCGGGATCTTTGTGGCCTTCTGGGCCTGCATGGTCGTTGTGGGAA
  TCTGCTACACCATCTTTGACCTGGGCTTTCGCTTTGATGTGGCATGGTTCCTGACGGAAACTTCCCCCTT
  CATGTGGTCGAATCTGGGCATTGGCCTAGCAATTTCTCTGTCTGTGGTTGGAGCAGCCTGGGGAATCTAT
  ATAACCGGCTCATCCATTATTGGGGGTGGGGTGAAAACCCCCAGAATCAAAACCAAGAACTTGGTTAGTA
  TTATCTTCTGTGAAGCGGTGGCCATCTATGGCATCATCATGGCAATTGTCATCAGCAACATGGCTGAGCC
  TTTCAGTGCTACGGAGCCCAAGGCCATTGGCCATCGAAACTACCATGCAGGTTACTCCATGTTTGGGGCT
  GGCCTCACAGTCGGTCTGTCCAACCTGTTCTGTGGAGTCTGCGTGGGCATCGTGGGCAGTGGGGCCGCCC
  TGGCAGATGCACAGAACCCCAGCCTCTTTGTAAAAATTCTCATCGTGGAGATCTTTGGCAGTGCCATTGG
  CCTCTTTGGGGTCATTGTTGCAATCCTTCAGACCTCCAGAGTGAAGATGGGTGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202139 representing NM_033617
  Red=Cloning site Green=Tags(s)
  
  MTGLELLYLGIFVAFWACMVVVGICYTIFDLGFRFDVAWFLTETSPFMWSNLGIGLAISLSVVGAAWGIY
  ITGSSIIGGGVKTPRIKTKNLVSIIFCEAVAIYGIIMAIVISNMAEPFSATEPKAIGHRNYHAGYSMFGA
  GLTVGLSNLFCGVCVGIVGSGAALADAQNPSLFVKILIVEIFGSAIGLFGVIVAILQTSRVKMGD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033617
• ORF Size: 615 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_033617.1, NP_291095.1
• RefSeq Size: 1009
• RefSeq ORF: 618
• Locus ID: 114143
• Gene Summary: Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. [UniProtKB/Swiss-Prot Function]