Eif4e (BC085087) Mouse Tagged ORF Clone

CAT#: MG202404

  • TrueORF®

Eif4e (GFP-tagged) - Mouse eukaryotic translation initiation factor 4E (cDNA clone MGC:103177 IMAGE:6438419)


  "BC085087" in other vectors (3)

Reconstitution Protocol

USD 300.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Eif4e
Synonyms Eif4e-ps, eIF-4E
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG202404 representing BC085087
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACTGTGGAACCGGAAACCACCCCTACCACTAATCCCCCACCTGCAGAAGAGGAAAAAACAGAGT
CTAATCAGGAGGTTGCTAACCCAGAGCACTATATTAAACACCCTCTACAGAACAGGTGGGCACTCTGGTT
TTTTAAAAATGATAAAAGCAAAACTTGGCAAGCAAACCTTCGATTGATCTCTAAGTTTGATACTGTTGAA
GACTTTTGGGCTCTATACAACCATATCCAGTTGTCTAGTAATTTAATGCCTGGCTGTGACTACTCACTTT
TTAAGGACGGGATTGAGCCTATGTGGGAAGATGAGAAAAACAAACGAGGAGGACGGTGGCTGATCACACT
GAACAAGCAGCAGAGACGGAGTGACCTCGATCGCTTCTGGCTAGAGACACTGCTGTGCCTTATTGGAGAA
TCTTTCGATGACTACAGTGATGATGTGTGTGGAGCTGTTGTTAATGTTAGAGCTAAAGGTGATAAGATAG
CAATATGGACTACTGAGTGTGAAAACAGAGATGCAGTCACACACATAGGGAGGGTATACAAGGAAAGGTT
AGGACTTCCTCCGAAGATAGTGATTGGTTATCAGTCCCACGCAGACACAGCTACAAAGAGCGGCTCCACC
ACTAAAAATAGGTTTGTTGTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG202404 representing BC085087
Red=Cloning site Green=Tags(s)

MATVEPETTPTTNPPPAEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVE
DFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGE
SFDDYSDDVCGAVVNVRAKGDKIAIWTTECENRDAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGST
TKNRFVV

TRTRRLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC085087
ORF Size 653 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq BC085087, AAH85087
RefSeq Size 2366
RefSeq ORF 653
Locus ID 13684
Gene Summary This gene encodes a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. It has also been associated with autism spectrum disorders. Consistently, knockout of this gene results in increased translation of neuroligins, postsynaptic proteins linked to autism spectrum disorders. Pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

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