Ebp (NM_007898) Mouse Tagged ORF Clone

CAT#: MG202773

Ebp (GFP-tagged) - Mouse phenylalkylamine Ca2+ antagonist (emopamil) binding protein (Ebp)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ebp
• Synonyms: AI255399; mSI; Pabp; SI; Td
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202773 representing NM_007898
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACCAATACGGTCCCCTTGCACCCGTACTGGCCCAGGCACCTGAAGCTGGACAACTTCGTGCCTA
  ATGACCTCCCGACTTCGCATATCCTGGTTGGCCTCTTCTCCATCTCTGGGGGCCTAATTGTGATCACGTG
  GCTGTTGTCTAGCCGAGCTTCCGTCGTCCCACTTGGAGCTGGGCGGCGACTGGCCTTGTGCTGGTTTGCT
  GTGTGTACCTTCATTCACCTTGTGATCGAGGGCTGGTTCTCTCTCTACAATGGCATCCTTTTAGAAGACC
  AAGCCTTCTTATCCCAACTCTGGAAAGAGTATTCCAAGGGAGATAGCCGATATATCCTTAGTGACAGCTT
  CGTCGTCTGTATGGAGACTGTCACAGCTTGTCTCTGGGGACCACTCAGCCTATGGGTAGTGATTGCCTTT
  CTCCGCCAACAGCCCTTCCGCTTTGTCCTACAGCTTGTGGTGTCTATGGGCCAGATATACGGGGATGTGC
  TGTACTTCCTGACAGAGCTACACGAAGGACTCCAGCATGGGGAGATAGGCCACCCCGTTTATTTCTGGTT
  CTATTTTGTTTTCCTGAATGCTGTATGGTTGGTGATACCAAGCATCCTTGTGCTTGATGCCATAAAGCAT
  CTCACTAGTGCCCAGAGCGTGCTGGACAGCAAAGTCATGAAAATTAAGAGCAAGCATAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202773 representing NM_007898
  Red=Cloning site Green=Tags(s)
  
  MTTNTVPLHPYWPRHLKLDNFVPNDLPTSHILVGLFSISGGLIVITWLLSSRASVVPLGAGRRLALCWFA
  VCTFIHLVIEGWFSLYNGILLEDQAFLSQLWKEYSKGDSRYILSDSFVVCMETVTACLWGPLSLWVVIAF
  LRQQPFRFVLQLVVSMGQIYGDVLYFLTELHEGLQHGEIGHPVYFWFYFVFLNAVWLVIPSILVLDAIKH
  LTSAQSVLDSKVMKIKSKHN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007898
• ORF Size: 690 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007898.2, NP_031924.1
• RefSeq Size: 1755
• RefSeq ORF: 693
• Locus ID: 13595
• Gene Summary: This gene encodes a transmembrane protein that localizes to the endoplasmic reticulum. This protein catalyses the conversion of delta8 to delta7 sterols, an important step in sterol biosynthesis. Mutations in this gene are responsible for the mouse tattered mutant phenotype. Tattered males are embryonic lethal, while heterozygous females have developmental defects. Deficiency of the related gene in human causes X-linked dominant chondrodysplasia punctata. [provided by RefSeq, May 2015]