Trmt12 (BC052533) Mouse Tagged ORF Clone

CAT#: MG203250

Trmt12 (GFP-tagged) - Mouse tRNA methyltranferase 12 homolog (S. cerevisiae) (cDNA clone MGC:63181 IMAGE:5255779)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trmt12
• Synonyms: 4632406N01Rik|AI449275|Tyw2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203250 representing BC052533
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGAGAGTGTGAGGAGTCTGTAGTTGTCGCAGTTGTGACTGAGCCCCGGTTCACGCAGAGATACC
  GAGACTACCTGGAGGAGCAGAAACTCTTGGACAGGCTGCACCGGGTGGCGAAGCTGCGGGATGGCGCGGT
  GGCTCTGCCGGTGCTGGCGGAAAGCCTTTCTGAGCAGCATCTGCAGGAGCTGAGGGATCGCGTGGCCCCA
  GGCAGCACTTGTGTGCTGACGCGGCTCCCGGATCCTCTTCCTTCCAAAAAGGCCCGGGTTCGTTCACCTG
  CTCAGATACTGTGTCTGGAGGTGAGGCGTTGGGTGGAGGACCGCGGAGTGACGTGGTCCGCTGAGTTGGA
  GGCAGATTTGCCCCGGTCTTGGCAACGGCATGGTGACCTAATGCTGCTTAGTGAAGACTGCTTCCAAGCC
  ACGCTGTGGAAAGGTCTGGAGCCAGAACTCTGGGAGACTGTTGCCTCGGCCCTGGGAGTTCAACGTTTGG
  CAAAACGAGGGCGGGTGCTACCTGATGGGACTCGAACTCCGTCAGTGACTCTGCTGCTGGGTGACCATGG
  CTGGGTAGAGCATATGGATAATGGCATCCGGTATAAGTTTGATGTGACACAGTGCATGTTCTCCTTTGGA
  AAACATCACCGAGAAACTTCGAGTGGCGTCTCTGTCCTGTGCTGGAGAGGTGCTGGTGGATCTCTACGCA
  GGGATTGGTTATTTTACATTGCCCTTCCTAGTTCATGCTGGCGCTGCCTTCGTACATGCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203250 representing BC052533
  Red=Cloning site Green=Tags(s)
  
  MERECEESVVVAVVTEPRFTQRYRDYLEEQKLLDRLHRVAKLRDGAVALPVLAESLSEQHLQELRDRVAP
  GSTCVLTRLPDPLPSKKARVRSPAQILCLEVRRWVEDRGVTWSAELEADLPRSWQRHGDLMLLSEDCFQA
  TLWKGLEPELWETVASALGVQRLAKRGRVLPDGTRTPSVTLLLGDHGWVEHMDNGIRYKFDVTQCMFSFG
  KHHRETSSGVSVLCWRGAGGSLRRDWLFYIALPSSCWRCLRTCL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052533
• ORF Size: 764 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC052533, AAH52533
• RefSeq Size: 1631
• RefSeq ORF: 764
• Locus ID: 68260