Etfbkmt (NM_177101) Mouse Tagged ORF Clone

CAT#: MG203272

Mettl20 (GFP-tagged) - Mouse RIKEN cDNA 4833442J19 gene (4833442J19Rik)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Etfbkmt
• Synonyms: 4833442J19Rik; AI256744; ETFB-KMT; Mettl20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203272 representing NM_177101
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTCAGCCTGTGTTGGAAAGCTCCCAGGAGCCCATGGAGTTTCCTGCAGGCTGTGAACAATGGGT
  CTCCCCTGTTTCTCTGGAGGACAGTGGGGAGCTGTTTGGATCCTAAGATGAAAGCCTACCTGGAAGAGAA
  CACTGAAGTCACCAGCAGTGGTAGCCTTACCCCGGAAATCCAGTTACGGCTTTTAACCCCCAGGTGCAAG
  TTTTGGTGGGAAAGGGCTGACCTGTGGCCCTACAGTGATCCCTACTGGGCTATTTACTGGCCAGGAGGCC
  AGGCTCTGTCTAGGTATCTTTTGGATAACCCTGCTGTTGTTAGAGGAAAGTCTGTCTTAGATCTTGGGAG
  TGGATGTGGAGCTACAGCCATTGCTGCCAAGATGAGTGGAGCATCAAAGATCTTGGCCAACGACATAGAC
  CCCATTGCAGGAATGGCGATTACACTAAATTGCAAATTAAATGGACTGAATCCTTTTCCTGTTTTAACTA
  AAAACATTTTGAATACCCAACAAGGTAAGTTTGATCTCATTGTTCTTGGAGATATGTTTTATGATGAAGA
  CCTTGCAGACAGTCTTCATCTGTGGCTGCAGAACTACTTTTGGACCCATGGAACCCGAGTACTGATTGGT
  GACCCTGGGAGGCCCCAGTTCAGTGGACATAGCATTCGGCATCAACTGTACCAACTAGTAGAATACACAC
  TTCCAGAGCCAACTCAGCAAGAGAACAATGGACTGACCACAAGTGCTGTATGGGATTTTCATCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203272 representing NM_177101
  Red=Cloning site Green=Tags(s)
  
  MAFSLCWKAPRSPWSFLQAVNNGSPLFLWRTVGSCLDPKMKAYLEENTEVTSSGSLTPEIQLRLLTPRCK
  FWWERADLWPYSDPYWAIYWPGGQALSRYLLDNPAVVRGKSVLDLGSGCGATAIAAKMSGASKILANDID
  PIAGMAITLNCKLNGLNPFPVLTKNILNTQQGKFDLIVLGDMFYDEDLADSLHLWLQNYFWTHGTRVLIG
  DPGRPQFSGHSIRHQLYQLVEYTLPEPTQQENNGLTTSAVWDFHP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177101
• ORF Size: 765 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177101.4, NP_796075.2
• RefSeq Size: 1478
• RefSeq ORF: 768
• Locus ID: 320204