Rchy1 (BC057143) Mouse Tagged ORF Clone

CAT#: MG203406

Rchy1 (GFP-tagged) - Mouse ring finger and CHY zinc finger domain containing 1 (cDNA clone MGC:67962 IMAGE:5249543)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rchy1
• Synonyms: mARNIP, Pirh2, ARNIP, CHIMP, PRO1996
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203406 representing BC057143
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGACGGCGCGGGAAGATGGCGTCCGCAACCTGGCGCAGGGGCCGCGGGGCTGCGAGCACTATG
  ACAGAGCTTGTCTCCTCAAGGCACCTTGTTGTGACAAGCTTTATACCTGCCGGCTGTGTCACGATACCAA
  TGAAGATCATCAGCTAGATCGTTTCAAAGTCAAGGAAGTGCAGTGCATCAACTGTGAAAAACTCCAACAT
  GCCCAGCAGACTTGTGAAGACTGTAGCACATTGTTTGGAGAATATTACTGCAGTATTTGCCACCTGTTTG
  ACAAAGATAAGAGGCAGTACCACTGTGAGAGCTGTGGGATTTGTAGGATTGGTCCAAAGGAAGATTTTTT
  CCATTGCTTGAAATGTAATTTATGCCTAACCACGAATCTTCGAGGAAAACACAAGTGTATTGAAAATGTT
  TCCCGGCAGAATTGTCCAATATGCTTGGAGGACATTCACACCTCCCGTGTTGTTGCTCACGTCTTGCCGT
  GTGGGCATCTCCTACATAGAACGTGTTATGAAGAAATGTTGAAAGAAGGTTACAGATGCCCATTGTGTAT
  GCATTCTGCCTTAGACATGACTCGGTACTGGAGACAACTAGATACTGAGGTAGCACAGACTCCCATGCCA
  TCCGAATACCAGAACGTGACTGTTGACATTCTCTGCAATGACTGTAATGGACGATCCACAGTCCAGTTCC
  ATATCTTAGGCATGAAATGTAAGCTTTGTGACTCCTACAATACTGCCCAAGCTGGAGGCCGTAGAGTGCC
  AGTGGATCAGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203406 representing BC057143
  Red=Cloning site Green=Tags(s)
  
  MAATAREDGVRNLAQGPRGCEHYDRACLLKAPCCDKLYTCRLCHDTNEDHQLDRFKVKEVQCINCEKLQH
  AQQTCEDCSTLFGEYYCSICHLFDKDKRQYHCESCGICRIGPKEDFFHCLKCNLCLTTNLRGKHKCIENV
  SRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKEGYRCPLCMHSALDMTRYWRQLDTEVAQTPMP
  SEYQNVTVDILCNDCNGRSTVQFHILGMKCKLCDSYNTAQAGGRRVPVDQQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC057143
• ORF Size: 785 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC057143, AAH57143
• RefSeq Size: 1989
• RefSeq ORF: 785
• Locus ID: 68098
• Gene Summary: This gene encodes a protein containing CHY-, CTCHY-, and RING-type zinc-fingers. The encoded protein functions as an E3 ubiquitin ligase, and mediates the degradation of target proteins such as p53. The activity of this protein is important in cell cycle regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]