Nfyc (BC053723) Mouse Tagged ORF Clone

CAT#: MG203685

Nfyc (GFP-tagged) - Mouse nuclear transcription factor-Y gamma (cDNA clone MGC:60675 IMAGE:30053020)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nfyc
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203685 representing BC053723
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACAGAAGGAGGGTTTGGCGGTACCAGCAGCAGTGATGCCCAGCAAAGCCTCCAGTCCTTCTGGC
  CCAGAGTCATGGAAGAAATCCGAAACTTAACAGTGAAAGATTTCCGAGTACAGGAACTCCCACTGGCTCG
  TATTAAGAAGATTATGAAACTGGATGAAGATGTGAAGATGATCAGTGCAGAAGCCCCTGTGCTGTTTGCT
  AAGGCAGCCCAGATTTTTATCACTGAGCTGACCCTTCGAGCCTGGATCCACACAGAGGATAACAAGCGTC
  GTACTCTTCAGAGGAATGATATTGCCATGGCAATTACAAAATTTGATCAGTTCGACTTTCTCATCGATAT
  TGTTCCAAGAGATGAACTGAAACCTCCAAAGCGCCAGGAGGAGGTACGCCAGTCTGTGACTCCTGCGGAG
  CCTGTCCAGTACTACTTCACGCTGGCTCAGCAGCCCACTGCTGTCCAGGTCCAGGGACAGCAGCAAGGCC
  AGCAGACCACCAGTTCTACGACCACCATCCAGCTTGGACAGATCATCATTGCACAGCCTCAGCAGGGTCA
  GACCACACCCGTGACCATGCAGGTTGGAGAAGGTCAGCAGGTGCAGATTGTACAGGCCCAACCTCAGGGT
  CAGGCCCAGCAGACCCAGAGTGGCACTGGACAGACCATGCAGGTGATGCAGCAGATCATTACCAACACAG
  GAGAGATCCAGCAGATCCCGGTGCAGCTGAATGCTGGCCAGCTGCAATATATCCGATTAGCCCAGCCTGT
  ATCAGGCACCCAAGTTGTCCAAGGACAGATCCAGACACTTGCTACCAATGCCCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203685 representing BC053723
  Red=Cloning site Green=Tags(s)
  
  MSTEGGFGGTSSSDAQQSLQSFWPRVMEEIRNLTVKDFRVQELPLARIKKIMKLDEDVKMISAEAPVLFA
  KAAQIFITELTLRAWIHTEDNKRRTLQRNDIAMAITKFDQFDFLIDIVPRDELKPPKRQEEVRQSVTPAE
  PVQYYFTLAQQPTAVQVQGQQQGQQTTSSTTTIQLGQIIIAQPQQGQTTPVTMQVGEGQQVQIVQAQPQG
  QAQQTQSGTGQTMQVMQQIITNTGEIQQIPVQLNAGQLQYIRLAQPVSGTQVVQGQIQTLATNAQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC053723
• ORF Size: 827 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC053723, AAH53723
• RefSeq Size: 1912
• RefSeq ORF: 827
• Locus ID: 18046
• Gene Summary: Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. [UniProtKB/Swiss-Prot Function]