Rnaseh1 (NM_011275) Mouse Tagged ORF Clone

CAT#: MG203888

Rnaseh1 (GFP-tagged) - Mouse ribonuclease H1 (Rnaseh1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rnaseh1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203888 representing NM_011275
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTGGCTGCTGCCGCTGTCCCGCACAGTGACACTGGCCGTCGTACGCCTGAGGCGAGGCATTTGCG
  GGCTCGGGATGTTCTATGCGGTGAGGAGAGGCCGCAGGACCGGAGTCTTCCTGAGTTGGAGTGAGTGCAA
  AGCCCAGGTGGACCGGTTTCCTGCTGCCAGGTTTAAGAAATTTGCCACAGAAGATGAGGCCTGGGCCTTT
  GTCAGGAGCTCTTCAAGCCCGGATGGTTCAAAAGGGCAGGAAAGTGCACATGAGCAGAAGTCACAGGTGA
  AGACCAGCAAGCGGCCTCGGGAGCCCCTGGGTGAAGGGGAAGAACTTCCAGAGCCAGGGCCAAAGCACAC
  ACGACAGGACACCGAGCCCGCTGCTGTAGTGAGCAAGGACACGTTTTCTTATATGGGAGAGTCAGTCATT
  GTCTACACGGATGGCTGTTGCTCCAGTAATGGACGGAAGCGGGCACGAGCAGGAATTGGCGTTTACTGGG
  GCCCAGGCCACCCCTTAAATGTAGGTATAAGGCTTCCTGGGCGACAGACAAACCAGAGGGCCGAGATCCA
  TGCAGCCTGCAAGGCCATCATGCAAGCCAAGGCTCAGAACATCAGCAAGCTGGTTCTGTACACAGACAGC
  ATGTTCACCATCAATGGGATAACTAACTGGGTTCAGGGCTGGAAGAAGAATGGCTGGAGAACAAGTACAG
  GGAAAGATGTGATCAACAAGGAGGACTTCATGGAGCTGGACGAGCTCACTCAGGGCATGGACATCCAATG
  GATGCACATTCCTGGTCACTCAGGATTTGTGGGCAATGAAGAGGCCGACAGACTGGCACGGGAAGGAGCG
  AAGCAGTCTGAGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203888 representing NM_011275
  Red=Cloning site Green=Tags(s)
  
  MRWLLPLSRTVTLAVVRLRRGICGLGMFYAVRRGRRTGVFLSWSECKAQVDRFPAARFKKFATEDEAWAF
  VRSSSSPDGSKGQESAHEQKSQVKTSKRPREPLGEGEELPEPGPKHTRQDTEPAAVVSKDTFSYMGESVI
  VYTDGCCSSNGRKRARAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIMQAKAQNISKLVLYTDS
  MFTINGITNWVQGWKKNGWRTSTGKDVINKEDFMELDELTQGMDIQWMHIPGHSGFVGNEEADRLAREGA
  KQSED
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011275
• ORF Size: 855 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011275.1, NP_035405.1
• RefSeq Size: 1430
• RefSeq ORF: 858
• Locus ID: 19819
• Gene Summary: This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which encodes 7aa in mouse. An alternately spliced transcript variant has been found which is a candidate for nonsense-mediated mRNA decay (NMD). [provided by RefSeq, Nov 2013]