Aqp4 (BC024526) Mouse Tagged ORF Clone

CAT#: MG204192

Aqp4 (GFP-tagged) - Mouse aquaporin 4 (cDNA clone MGC:37514 IMAGE:4985265)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Aqp4
• Synonyms: MIWC, mMIWC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204192 representing BC024526
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCTTTCAAAGGAGTCTGGACTCAGGCTTTCTGGAAGGCAGTCTCAGCAGAATTTCTGGCCACGC
  TTATCTTTGTTTTGCTCGGTGTGGGATCCACCATAAACTGGGGTGGCTCAGAAAACCCCTTACCTGTGGA
  CATGGTCCTCATCTCCCTTTGCTTTGGACTCAGCATTGCTACCATGGTGCAGTGCTTTGGCCACATCAGT
  GGTGGCCACATCAATCCCGCTGTGACTGTAGCCATGGTGTGCACACGAAAGATCAGCATCGCTAAGTCCG
  TCTTCTACATCATTGCACAGTGCCTGGGGGCCATCATTGGAGCCGGCATCCTCTACCTGGTCACACCTCC
  CAGTGTGGTTGGAGGATTGGGAGTCACCACGGTTCATGGAAACCTCACCGCTGGCCATGGGCTCCTGGTG
  GAGTTAATAATCACTTTCCAGTTGGTGTTCACTATTTTTGCCAGCTGTGATTCCAAACGAACTGATGTTA
  CTGGTTCAATAGCTTTAGCAATTGGATTTTCCGTTGCAATTGGACATTTGTTTGCAATCAATTATACTGG
  AGCCAGCATGAATCCAGCTCGATCTTTTGGACCCGCAGTTATCATGGGAAACTGGGCAAACCACTGGATA
  TATTGGGTTGGACCAATCATGGGCGCTGTGCTGGCAGGTGCCCTTTATGAGTATGTCTTCTGTCCTGATG
  TGGAGCTCAAACGTCGCCTTAAGGAAGCCTTCAGCAAAGCCGCGCAGCAGACAAAAGGGAGCTACATGGA
  GGTGGAGGACAACCGGAGCCAAGTGGAGACGGAAGACTTGATCCTGAAGCCCGGAGTGGTGCATGTGATT
  GACATTGACCGTGGAGAAGAGAAGAAGGGGAAAGACTCTTCGGGAGAGGTATTGTCTTCCGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204192 representing BC024526
  Red=Cloning site Green=Tags(s)
  
  MVAFKGVWTQAFWKAVSAEFLATLIFVLLGVGSTINWGGSENPLPVDMVLISLCFGLSIATMVQCFGHIS
  GGHINPAVTVAMVCTRKISIAKSVFYIIAQCLGAIIGAGILYLVTPPSVVGGLGVTTVHGNLTAGHGLLV
  ELIITFQLVFTIFASCDSKRTDVTGSIALAIGFSVAIGHLFAINYTGASMNPARSFGPAVIMGNWANHWI
  YWVGPIMGAVLAGALYEYVFCPDVELKRRLKEAFSKAAQQTKGSYMEVEDNRSQVETEDLILKPGVVHVI
  DIDRGEEKKGKDSSGEVLSSV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC024526
• ORF Size: 905 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC024526, AAH24526
• RefSeq Size: 1622
• RefSeq ORF: 905
• Locus ID: 11829
• Gene Summary: This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015]