Dpagt1 (BC003943) Mouse Tagged ORF Clone

CAT#: MG204217

Dpagt1 (GFP-tagged) - Mouse dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dpagt1
• Synonyms: AU021132|Dpagt2|GPT|Gnpta
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204217 representing BC003943
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTTCCTGGGGTTTGCTGATGATGTCCTCAATCTCCGCTGGCGCCACAAGCTGCTGCTGCCCACAG
  CTGCCTCACTACCTCTCCTCATGGTCTACTTCACAAACTTTGGCAATACAACCATCGTGGTGCCCAAGCC
  CTTCCGCTGGATTCTGGGCCTGCATTTGGACTTGGGGATCCTGTACTACGTCTACATGGGGCTGCTTGCA
  GTGTTCTGTACCAATGCCATCAACATCCTGGCGGGCATTAATGGCCTAGAGGCCGGTCAGTCACTAGTCA
  TCTCTGCTTCTATCATTGTCTTCAACCTGGTGGAACTGGAAGGTGATTATCGAGATGATCATATCTTTTC
  CCTTTACTTCATGATACCATTTTTCTTTACCACCTTGGGACTGCTTTACCACAACTGGTACCCGTCCCGC
  GTGTTTGTGGGAGACACCTTCTGTTACTTTGCGGGCATGACTTTTGCCGTGGTGGGGATCTTGGGACACT
  TCAGCAAGACCATGCTGCTCTTCTTTATGCCACAAGTATTCAATTTCCTCTACTCACTGCCTCAGCTCTT
  CCATATCATCCCCTGCCCTCGACACCGGATGCCCAGACTCAACGCAAAGACAGGCAAACTGGAAATGAGC
  TATTCCAAGTTCAAGACCAAGAACCTCTCTTTCCTGGGCACCTTTATTTTAAAGGTAGCAGAGAACCTCC
  GGTTAGTGACAGTTCACCAAGGTGAGAGTGAGGACGGTGCCTTCACTGAGTGTAACAACATGACCCTCAT
  CAACTTGCTACTTAAAGTCTTTGGGCCTATACATGAGAGAAACCTCACCCTGCTCCTGCTGCTCCTGCAG
  GTCCTAAGCAGCGCCGCCACCTTCTCCATTCGTTACCAACTCGTCCGACTCTTCTATGATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204217 representing BC003943
  Red=Cloning site Green=Tags(s)
  
  MIFLGFADDVLNLRWRHKLLLPTAASLPLLMVYFTNFGNTTIVVPKPFRWILGLHLDLGILYYVYMGLLA
  VFCTNAINILAGINGLEAGQSLVISASIIVFNLVELEGDYRDDHIFSLYFMIPFFFTTLGLLYHNWYPSR
  VFVGDTFCYFAGMTFAVVGILGHFSKTMLLFFMPQVFNFLYSLPQLFHIIPCPRHRMPRLNAKTGKLEMS
  YSKFKTKNLSFLGTFILKVAENLRLVTVHQGESEDGAFTECNNMTLINLLLKVFGPIHERNLTLLLLLLQ
  VLSSAATFSIRYQLVRLFYDV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC003943
• ORF Size: 906 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC003943.1
• RefSeq Size: 1639
• RefSeq ORF: 905
• Locus ID: 13478
• Gene Summary: Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides. [UniProtKB/Swiss-Prot Function]