Sparc (BC004638) Mouse Tagged ORF Clone

CAT#: MG204230

Sparc (GFP-tagged) - Mouse secreted acidic cysteine rich glycoprotein (cDNA clone MGC:6232 IMAGE:3586402)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sparc
• Synonyms: BM-40
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204230 representing BC004638
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGCCCTGGCAGCCCCTCAGCAGACTGAAG
  TTGCTGAGGAGATAGTGGAGGAGGAAACCGTGGTGGAGGAGACAGGGGTACCTGTGGGTGCCAACCCAGT
  CCAGGTGGAAATGGGAGAATTTGAGGACGGTGCAGAGGAAACGGTCGAGGAGGTGGTGGCTGACAACCCC
  TGCCAGAACCATCATTGCAAACATGGCAAGGTGTGTGAGCTGGACGAGAGCAACACCCCCATGTGTGTGT
  GCCAGGACCCCACCAGCTGCCCTGCTCCCATTGGCGAGTTTGAGAAGGTATGCAGCAATGACAACAAGAC
  CTTCGACTCTTCCTGCCACTTCTTTGCCACCAAGTGCACCCTGGAGGGCACCAAGAAGGGCCACAAGCTC
  CACCTGGACTACATCGGACCATGCAAATACATCGCCCCCTGCCTGGATTCCGAGCTGACCGAATTCCCTC
  TGCGCATGCGTGACTGGCTCAAAAATGTCCTGGTCACCTTGTACGAGAGAGATGAGGGCAACAACCTCCT
  CACTGAGAAGCAGAAGCTGCGTGTGAAGAAGATCCATGAGAATGAGAAGCGCCTGGAGGCTGGAGACCAC
  CCCGTGGAGCTGTTGGCCCGAGACTTTGAGAAGAACTACAATATGTACATCTTCCCTGTCCACTGGCAGT
  TTGGCCAGCTGGATCAGCACCCTATTGATGGGTACCTGTCCCACACTGAGCTGGCCCCACTGCGTGCTCC
  CCTCATCCCCATGGAACATTGCACCACACGTTTCTTTGAGACCTGTGACCTAGACAACGACAAGTACATT
  GCCCTGGAGGAATGGGCCGGCTGCTTTGGCATCAAGGAGCAGGACATCAACAAGGATCTGGTGATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204230 representing BC004638
  Red=Cloning site Green=Tags(s)
  
  MRAWIFFLLCLAGRALAAPQQTEVAEEIVEEETVVEETGVPVGANPVQVEMGEFEDGAEETVEEVVADNP
  CQNHHCKHGKVCELDESNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKL
  HLDYIGPCKYIAPCLDSELTEFPLRMRDWLKNVLVTLYERDEGNNLLTEKQKLRVKKIHENEKRLEAGDH
  PVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYI
  ALEEWAGCFGIKEQDINKDLVI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC004638
• ORF Size: 908 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC004638, AAH04638
• RefSeq Size: 1153
• RefSeq ORF: 908
• Locus ID: 20692
• Gene Summary: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. [UniProtKB/Swiss-Prot Function]