Hadh (NM_008212) Mouse Tagged ORF Clone

CAT#: MG204244

Hadh (GFP-tagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase (Hadh)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hadh
• Synonyms: AA409008; AU019341; AW742602; Hadhsc; HCDH; Schad
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204244 representing NM_008212
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTCGTGACCAGGCAATTCTTGCGCTCCATGTCCTCCTCTTCCTCTGCGTCGGCGGCCGCCAAGA
  AGATCCTGATCAAGCATGTGACCGTCATCGGCGGCGGGCTGATGGGCGCCGGCATCGCGCAGGTAGCTGC
  AGCAACTGGCCATACAGTAGTATTGGTGGACCAAACGGAAGACATCCTGGCAAAATCCAAGAAGGGAATT
  GAGGAGAGTCTAAAGAGGATGGCAAAGAAGAAGTTCACAGAAAACCCTAAGGCTGGAGATGAGTTTGTGG
  AGAAGACCCTGAGCTGCCTTTCAACCAGCACCGATGCGGCATCCGTGGTGCACAGCACAGACCTGGTGGT
  GGAGGCCATTGTGGAGAACCTGAAGCTGAAGAACGAGCTGTTCCAGAGGCTGGACAAGTTCGCTGCAGAA
  CACACCATCTTTGCCAGCAACACGTCTTCTTTGCAGATCACAAACATAGCCAATGCCACCACCAGACAAG
  ACCGATTTGCTGGCCTCCACTTTTTCAACCCCGTGCCTATGATGAAGCTTGTGGAGGTCATCAAAACACC
  GATGACCAGCCAGAAGACATTTGAATCTCTGGTGGACTTTTGTAAAACCTTAGGAAAGCATCCTGTCTCT
  TGCAAGGACACTCCTGGATTCATCGTGAACCGACTCTTGGTGCCATACCTCATTGAGGCCGTCAGGCTAC
  ACGAGCGAGGCGATGCGTCTAAGGAAGACATCGATACAGCGATGAAGCTGGGAGCTGGGTATCCCATGGG
  TCCGTTTGAGCTTCTTGACTATGTTGGACTGGATACTACAAAGTTCATCTTGGACGGGTGGCATGAAATG
  GAGCCAGAGAACCCCTTATTTCAGCCCAGTCCTTCCATGAATAATCTGGTGGCCCAGAAGAAGCTGGGCA
  AGAAGACCGGAGAAGGATTTTACAAATACAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204244 representing NM_008212
  Red=Cloning site Green=Tags(s)
  
  MAFVTRQFLRSMSSSSSASAAAKKILIKHVTVIGGGLMGAGIAQVAAATGHTVVLVDQTEDILAKSKKGI
  EESLKRMAKKKFTENPKAGDEFVEKTLSCLSTSTDAASVVHSTDLVVEAIVENLKLKNELFQRLDKFAAE
  HTIFASNTSSLQITNIANATTRQDRFAGLHFFNPVPMMKLVEVIKTPMTSQKTFESLVDFCKTLGKHPVS
  CKDTPGFIVNRLLVPYLIEAVRLHERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFILDGWHEM
  EPENPLFQPSPSMNNLVAQKKLGKKTGEGFYKYK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008212
• ORF Size: 942 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008212.2, NP_032238.2
• RefSeq Size: 1721
• RefSeq ORF: 945
• Locus ID: 15107
• Gene Summary: Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA. [UniProtKB/Swiss-Prot Function]