Rdh12 (BC016204) Mouse Tagged ORF Clone

CAT#: MG204254

Rdh12 (GFP-tagged) - Mouse retinol dehydrogenase 12 (cDNA clone MGC:27665 IMAGE:4527851)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rdh12
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204254 representing BC016204
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTTTATCTTGGTACTGCTTACGTCCTTCCTCTCCATCTTGTATCTGACAGCTCCATCCATCAGGA
  AGTTCTTTGCTGGTGGAGTTTGTACAACAAATGTCCAGATCCCAGGGAAGGTAGTGGTCATCACAGGTGC
  CAACACAGGCATTGGCAAGGAGACAGCCAGAGAGCTTGCTCGAAGAGGAGCACGAGTATACATTGCTTGC
  CGAGATGTGCTGAAGGGAGAGTCTGCTGCTAGTGAAATCCGAGCAGATACCAAGAACTCCCAGGTGCTAG
  TGCGGAAATTGGACCTGTCTGACACCAAATCCATCCGAGCCTTTGCTGAACGCTTCCTAGCAGGAGTGAT
  GATGTGCCCATATTCTAAGACAACAGATGGCTTTGAGACCCACTTTGGAGTCAACCACCTGGGACACTTT
  CTTCTTACATACCTGCTGTTGGAGAGGCTGAAGGAGTCTGCTCCCGCACGGGTGGTCAACCTTTCCTCAA
  TAGCTCACCTGATTGGCAAGATCCGTTTCCATGACCTCCAAGGCCAGAAACGATACTGCAGTGCTTTTGC
  CTATGGCCACAGCAAGCTGGCCAATCTGCTCTTCACTCGAGAACTGGCTAAGCGGCTCCAAGGGACTGGA
  GTCACCGCCTATGCGGTTCACCCGGGCGTTGTATTGTCAGAGATCACCAGGAACTCCTACCTGCTGTGTT
  TGCTATGGCGGCTCTTCTCACCCTTCTTCAAGTCCACTTCTCAGGGGGCTCAGACCAGCCTGCACTGTGC
  TCTGGCGGAGGACCTAGAGCCCCTGAGTGGAAAGTACTTCAGTGACTGCAAGAGGATGTGGGTATCCTCA
  AGGGCCCGGAACAAGAAAACAGCTGAGCGATTGTGGAACGTCAGCTGCGAGCTTCTAGGAATCCAGTGGG
  AA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204254 representing BC016204
  Red=Cloning site Green=Tags(s)
  
  MLFILVLLTSFLSILYLTAPSIRKFFAGGVCTTNVQIPGKVVVITGANTGIGKETARELARRGARVYIAC
  RDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAERFLAGVMMCPYSKTTDGFETHFGVNHLGHF
  LLTYLLLERLKESAPARVVNLSSIAHLIGKIRFHDLQGQKRYCSAFAYGHSKLANLLFTRELAKRLQGTG
  VTAYAVHPGVVLSEITRNSYLLCLLWRLFSPFFKSTSQGAQTSLHCALAEDLEPLSGKYFSDCKRMWVSS
  RARNKKTAERLWNVSCELLGIQWE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC016204
• ORF Size: 914 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC016204, AAH16204
• RefSeq Size: 1659
• RefSeq ORF: 914
• Locus ID: 77974
• Gene Summary: The protein encoded by this gene is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. The encoded enzyme also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Defects in the human gene are associated with Leber congenital amaurosis type 13, and Retinitis Pigmentosa 53. [provided by RefSeq, Sep 2015]