Rdh12 (BC016204) Mouse Tagged ORF Clone
CAT#: MR204254
- TrueORF®
Rdh12 (Myc-DDK-tagged) - Mouse retinol dehydrogenase 12 (cDNA clone MGC:27665 IMAGE:4527851)
"BC016204" in other vectors (4)
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Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Rdh12 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204254 representing BC016204
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTTTATCTTGGTACTGCTTACGTCCTTCCTCTCCATCTTGTATCTGACAGCTCCATCCATCAGGA AGTTCTTTGCTGGTGGAGTTTGTACAACAAATGTCCAGATCCCAGGGAAGGTAGTGGTCATCACAGGTGC CAACACAGGCATTGGCAAGGAGACAGCCAGAGAGCTTGCTCGAAGAGGAGCACGAGTATACATTGCTTGC CGAGATGTGCTGAAGGGAGAGTCTGCTGCTAGTGAAATCCGAGCAGATACCAAGAACTCCCAGGTGCTAG TGCGGAAATTGGACCTGTCTGACACCAAATCCATCCGAGCCTTTGCTGAACGCTTCCTAGCAGGAGTGAT GATGTGCCCATATTCTAAGACAACAGATGGCTTTGAGACCCACTTTGGAGTCAACCACCTGGGACACTTT CTTCTTACATACCTGCTGTTGGAGAGGCTGAAGGAGTCTGCTCCCGCACGGGTGGTCAACCTTTCCTCAA TAGCTCACCTGATTGGCAAGATCCGTTTCCATGACCTCCAAGGCCAGAAACGATACTGCAGTGCTTTTGC CTATGGCCACAGCAAGCTGGCCAATCTGCTCTTCACTCGAGAACTGGCTAAGCGGCTCCAAGGGACTGGA GTCACCGCCTATGCGGTTCACCCGGGCGTTGTATTGTCAGAGATCACCAGGAACTCCTACCTGCTGTGTT TGCTATGGCGGCTCTTCTCACCCTTCTTCAAGTCCACTTCTCAGGGGGCTCAGACCAGCCTGCACTGTGC TCTGGCGGAGGACCTAGAGCCCCTGAGTGGAAAGTACTTCAGTGACTGCAAGAGGATGTGGGTATCCTCA AGGGCCCGGAACAAGAAAACAGCTGAGCGATTGTGGAACGTCAGCTGCGAGCTTCTAGGAATCCAGTGGG AA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204254 representing BC016204
Red=Cloning site Green=Tags(s) MLFILVLLTSFLSILYLTAPSIRKFFAGGVCTTNVQIPGKVVVITGANTGIGKETARELARRGARVYIAC RDVLKGESAASEIRADTKNSQVLVRKLDLSDTKSIRAFAERFLAGVMMCPYSKTTDGFETHFGVNHLGHF LLTYLLLERLKESAPARVVNLSSIAHLIGKIRFHDLQGQKRYCSAFAYGHSKLANLLFTRELAKRLQGTG VTAYAVHPGVVLSEITRNSYLLCLLWRLFSPFFKSTSQGAQTSLHCALAEDLEPLSGKYFSDCKRMWVSS RARNKKTAERLWNVSCELLGIQWE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC016204 |
ORF Size | 912 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC016204, AAH16204 |
RefSeq Size | 1659 bp |
RefSeq ORF | 914 bp |
Locus ID | 77974 |
MW | 60.8 kDa |
Gene Summary | The protein encoded by this gene is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. The encoded enzyme also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Defects in the human gene are associated with Leber congenital amaurosis type 13, and Retinitis Pigmentosa 53. [provided by RefSeq, Sep 2015] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217923 | Rdh12 (untagged) - Mouse retinol dehydrogenase 12 (cDNA clone MGC:27665 IMAGE:4527851), (10ug) |
USD 730.00 |
|
MG204254 | Rdh12 (GFP-tagged) - Mouse retinol dehydrogenase 12 (cDNA clone MGC:27665 IMAGE:4527851) |
USD 300.00 |
|
MR204254L3 | Lenti ORF clone of Rdh12 (Myc-DDK-tagged) - Mouse retinol dehydrogenase 12 (cDNA clone MGC:27665 IMAGE:4527851) |
USD 500.00 |
|
MR204254L4 | Lenti ORF clone of Rdh12 (mGFP-tagged) - Mouse retinol dehydrogenase 12 (cDNA clone MGC:27665 IMAGE:4527851) |
USD 500.00 |
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