Atp1b1 (BC027319) Mouse Tagged ORF Clone

CAT#: MG204262

Atp1b1 (GFP-tagged) - Mouse ATPase, Na+/K+ transporting, beta 1 polypeptide (cDNA clone MGC:6286 IMAGE:2648940)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atp1b1
• Synonyms: Atp4b|Atpb|Atpb-1|NKbeta1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204262 representing BC027319
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGCGGAAAAGCCAAGGAGGAAGGCAGCTGGAAGAAATTCATCTGGAACTCGGAGAAGAAGGAGT
  TTTTGGGCAGGACCGGTGGTAGTTGGTTTAAGATCCTTCTGTTCTACGTGATATTTTATGGCTGCCTGGC
  TGGCATCTTCATCGGGACCATCCAAGTAATGCTGCTAACCATCAGTGAACTGAAGCCCACATACCAGGAC
  CGAGTGGCCCCGCCAGGATTGACACAGATTCCCCAGATCCAGAAGACTGAGATCTCCTTCCGTCCTAATG
  ACCCCAAGAGCTACGAGGCCTACGTGCTAAACATCATCAGGTTCCTGGAAAAGTACAAGGATTCAGCCCA
  GAAGGACGACATGATTTTCGAGGACTGTGGCAATGTTCCCAGTGAACCCAAGGAACGGGGCGACATCAAT
  CACGAACGAGGAGAGAGGAAGGTGTGCAGGTTCAAGCTTGACTGGCTGGGGAACTGCTCCGGTCTCAATG
  ATGACTCTTACGGCTACAGAGAGGGGAAGCCCTGCATCATTATCAAGCTCAACCGAGTGTTGGGCTTCAA
  ACCGAAGCCTCCCAAGAATGAATCCTTGGAGACTTACCCACTGATGATGAAGTATAATCCAAATGTCCTG
  CCTGTTCAGTGCACTGGCAAGAGAGATGAAGATAAGGATAAAGTCGGGAACATAGAGTACTTTGGGATGG
  GCGGATACTACGGCTTCCCTCTGCAGTATTATCCCTACTACGGCAAACTCCTGCAGCCCAAGTACCTGCA
  GCCCCTGCTGGCCGTGCAGTTCACCAACCTCACCGTGGACACTGAAATCCGCGTCGAGTGTAAGGCGTAT
  GGTGAGAACATTGGGTACAGTGAGAAAGACCGTTTTCAGGGACGCTTTGATGTAAAAATTGAAATTAAGA
  GC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204262 representing BC027319
  Red=Cloning site Green=Tags(s)
  
  MARGKAKEEGSWKKFIWNSEKKEFLGRTGGSWFKILLFYVIFYGCLAGIFIGTIQVMLLTISELKPTYQD
  RVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIIRFLEKYKDSAQKDDMIFEDCGNVPSEPKERGDIN
  HERGERKVCRFKLDWLGNCSGLNDDSYGYREGKPCIIIKLNRVLGFKPKPPKNESLETYPLMMKYNPNVL
  PVQCTGKRDEDKDKVGNIEYFGMGGYYGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTVDTEIRVECKAY
  GENIGYSEKDRFQGRFDVKIEIKS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC027319
• ORF Size: 914 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC027319, AAH27319
• RefSeq Size: 2233
• RefSeq ORF: 914
• Locus ID: 11931
• Gene Summary: This gene encodes an integral membrane protein that comprises a subunit of an ATP-metabolizing enzyme responsible for transporting sodium and potassium ions across the plasma membrane. This enzyme regulates the electrochemical gradient of these ions in cells, and plays a central role in osmoregulation and signal transmission in nerves and muscles, among other biological processes. The encoded protein is the non-catalytic beta subunit; it works together with a catalytic alpha subunit and a gamma subunit. [provided by RefSeq, Mar 2013]