Bsnd (NM_080458) Mouse Tagged ORF Clone

CAT#: MG204322

Bsnd (GFP-tagged) - Mouse Bartter syndrome, infantile, with sensorineural deafness (Barttin) (Bsnd)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bsnd
• Synonyms: MGC47291
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204322 representing NM_080458
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGATGAGAAGACCTTCCGAATCGGCTTCATCGTGCTGGGCCTCTTCCTGCTATCCCTGGGTACAT
  TCCTTATGAGCCACGATCGACCCCAGGTCTACGGCACCTTCTATGCCATGGGCAGCGTCATGGTGATCGG
  GGGTGTCATCTGGAGCATGTGCCAATGCTATCCTAAGATCACCTTTGTGCCAGCAGACTCTGACTTCCAA
  GGCATCTTGTCCCCAAAGGCCCTGAGCCTGCTGGAGACCGGGCTTTCGGAGGTAAAGAGCCCCCAGCCCC
  CCTACGTCAGGCTGTGGGAAGAAGCCGCCTATGACCAGAGCCTCCCAGACTTCACTCACATCCAAATGAA
  GGTCATGGGCTACAGTGAGGACCCCAGGCCACTGCTGGCCCCCGAGCTGAAGACAGGAGCCAGCAGTGTC
  AGGGAAGGCGAGCCTCGTACTGCTCAGGCCTGGATGGAGGCTCCGGTGGTCGTCCATAGAGGGTCAGATG
  AGAACGAGGGAGAGAAATCCCATTCTCAGAGCAGCCCTTCAGTGGGTCCCCAAGGCTCTGCACCCTTGGC
  CTCATTCCATGACGACCTGGACGTGGGCTCCAGCGAAGGCAGCAGCCTGCAACCATCCCCCAACAGGGAT
  GAGCCACACCGGCAGGTGCCCTGGGCCAGCAGGGGTCCACTGGATCGCTTCAGTGACTTTGCTCTGATTG
  ACGACACCCCTACATCTGAGGACACGGTCCTGGACGGGCAGGCACGGGAGGCAGCGCTACCCAGAAAGCA
  GCAATGGTCCCTGAGGATGAAGGGGGAGACTGTCCAGGCACGCGCAGAAGAACCGGAGCAGGAGGAGGAA
  GATCTGTATTACGGGCTGCCAGACAGCCCTGGGGACCCCCTACCCGACAAAGAGTTGGGCTTCGAGCCTG
  ACATCCAGGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204322 representing NM_080458
  Red=Cloning site Green=Tags(s)
  
  MADEKTFRIGFIVLGLFLLSLGTFLMSHDRPQVYGTFYAMGSVMVIGGVIWSMCQCYPKITFVPADSDFQ
  GILSPKALSLLETGLSEVKSPQPPYVRLWEEAAYDQSLPDFTHIQMKVMGYSEDPRPLLAPELKTGASSV
  REGEPRTAQAWMEAPVVVHRGSDENEGEKSHSQSSPSVGPQGSAPLASFHDDLDVGSSEGSSLQPSPNRD
  EPHRQVPWASRGPLDRFSDFALIDDTPTSEDTVLDGQAREAALPRKQQWSLRMKGETVQARAEEPEQEEE
  DLYYGLPDSPGDPLPDKELGFEPDIQG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080458
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_080458.2, NP_536706.2
• RefSeq Size: 2716
• RefSeq ORF: 924
• Locus ID: 140475
• Gene Summary: Functions as a beta-subunit for CLCNKA and CLCNKB chloride channels. In the kidney CLCNK/BSND heteromers mediate chloride reabsorption by facilitating its basolateral efflux. In the stria, CLCNK/BSND channels drive potassium secretion by recycling chloride for the basolateral SLC12A2 cotransporter. [UniProtKB/Swiss-Prot Function]