Tpm2 (BC014809) Mouse Tagged ORF Clone

CAT#: MG204354

Tpm2 (GFP-tagged) - Mouse tropomyosin 2, beta (cDNA clone MGC:18587 IMAGE:3497670)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tpm2
• Synonyms: Tpm-2|Trop-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204354 representing BC014809
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCCATCAAGAAGAAGATGCAGATGCTGAAACTGGACAAGGAGAATGCCATCGACCGCGCGGAGC
  AGGCCGAAGCCGACAAAAAGCAAGCTGAAGACCGATGCAAGCAGCTGGAGGAAGAGCAGCAGGCCCTCCA
  GAAGAAGCTGAAGGGGACAGAGGACGAGGTGGAAAAGTATTCCGAGTCCGTGAAGGATGCCCAGGAGAAA
  CTGGAGCAGGCTGAGAAGAAGGCCACCGACGCTGAAGCAGATGTGGCCTCTCTGAACCGCCGCATTCAGC
  TCGTAGAGGAGGAGTTGGATCGGGCACAGGAGCGCCTGGCTACAGCCTTGCAAAAGCTGGAGGAGGCTGA
  GAAAGCCGCGGATGAGAGCGAGAGAGGAATGAAGGTCATTGAAAACCGGGCCATGAAGGATGAGGAAAAG
  ATGGAGCTGCAGGAGATGCAGCTGAAGGAAGCCAAGCACATCGCTGAGGACTCAGACCGCAAATATGAGG
  AGGTGGCCAGGAAGCTGGTGATCCTGGAAGGGGAGCTGGAGCGCTCGGAAGAGAGAGCCGAGGTGGCTGA
  GAGCCGAGCCAGACAGCTGGAGGAGGAGCTTCGAACCATGGACCAAGCCCTCAAGTCGCTGATAGCCTCA
  GAGGAGGAGGTAGTAGCCTCTCTGGGCCTTTCTAGGCAATGGCACCTTTTCTCACTCACCTCACGTCCCT
  CCCCAGTGCAGCGTCTCAGGCTACTGTCTCTCTCCTGCAGCAGGAGCGGGGCGGGGGCAGGGCTTCCAAA
  CTCAGCCTGCTGTCACTCTTCTCTCACCACTTTGCTCTTCTCTTTTCTCCTCCGCCCCTCCCCCACTGTG
  CCACCCCATCCCCCCCCCCCCCGCCCGCCCCACTGCCTCACCTCACTGTGCCCTCACGCTCCATCCTGCC
  ACACGCCCCTGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204354 representing BC014809
  Red=Cloning site Green=Tags(s)
  
  MDAIKKKMQMLKLDKENAIDRAEQAEADKKQAEDRCKQLEEEQQALQKKLKGTEDEVEKYSESVKDAQEK
  LEQAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEK
  MELQEMQLKEAKHIAEDSDRKYEEVARKLVILEGELERSEERAEVAESRARQLEEELRTMDQALKSLIAS
  EEEVVASLGLSRQWHLFSLTSRPSPVQRLRLLSLSCSRSGAGAGLPNSACCHSSLTTLLFSFLLRPSPTV
  PPHPPPPRPPHCLTSLCPHAPSCHTPLQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC014809
• ORF Size: 926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC014809, AAH14809
• RefSeq Size: 1561
• RefSeq ORF: 926
• Locus ID: 22004
• Gene Summary: This gene belongs to the tropomyosin family which encodes proteins that bind to actin filaments and stabilize them by regulating access to actin modifying proteins. The encoded protein is a high molecular weight tropomyosin expressed in slow skeletal muscle. In humans, mutations in this gene are associated with nemaline myopathy, cap disease and distal arthrogryposis syndromes. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2013]