Bpnt1 (BC011036) Mouse Tagged ORF Clone

CAT#: MG204360

Bpnt1 (GFP-tagged) - Mouse bisphosphate 3'-nucleotidase 1 (cDNA clone MGC:13732 IMAGE:4160742)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bpnt1
• Synonyms: BPntase
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204360 representing BC011036
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCAGCCACACCGTGTTGATGCGGCTGGTAGCTTCAGCATATTCTATCGCTCAAAAGGCAGGAA
  CCATAGTCAGGTGTGTCATTGCTGAAGGAGACCTGGGCATCGTGCAGAAGACCTCGGCCACCGACCTGCA
  GACCAAAGCTGACCGCTTGGTGCAGATGAGCATATGCTCTTCCCTGGCCCGGAAGTTCCCGAAGCTGACC
  ATCATAGGGGAAGAGGACCTGCCTCCTGGGGAAGTGGATCAAGAGCTGATTGAAGACGGGCAGTGGGAGG
  AGATCCTGAAGCAGCCGTGCCCCTCGCAGTACAGTGCTATCAAGGAGGAAGACCTTGTGGTTTGGGTTGA
  CCCCTTAGATGGCACCAAGGAGTACACTGAAGGTCTTCTTGACAATGTAACAGTTCTCATTGGGATTGCT
  TATGAAGGAAAGGCCATCGCAGGCATCATCAACCAGCCGTATTACAACTACCAGGCAGGACCCGACGCGG
  TGCTGGGCAGGACCATCTGGGGAGTTCTGGGTTTGGGTGCTTTTGGGTTTCAGCTGAAGGAAGCCCCTGC
  CGGGAAGCGCATTATCACAACCACCAGATCCCATAGCAACCAGCTGGTCACAGACTGCATTTCCGCCATG
  AACCCCGACACCGTGCTGCGAGTGGGAGGAGCAGGAAACAAGATTATCCAGCTGATCGAAGGCAAAGCTT
  CTGCCTATGTGTTTGCAAGTCCTGGGTGTAAGAAGTGGGACACTTGTGCCCCAGAAGTTATCTTGCATGC
  TGTGGGAGGGAAGTTGACAGACATCCATGGGAATGCCCTGCAGTACAACAAGGAGGTGAAACATATGAAC
  TCTGCCGGTGTCCTGGCTGCACTGAGGAACTACGAGTACTATGCAAGCCATGTTCCAGAGTCGGTCAAAA
  ATGCGCTCATTCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204360 representing BC011036
  Red=Cloning site Green=Tags(s)
  
  MASSHTVLMRLVASAYSIAQKAGTIVRCVIAEGDLGIVQKTSATDLQTKADRLVQMSICSSLARKFPKLT
  IIGEEDLPPGEVDQELIEDGQWEEILKQPCPSQYSAIKEEDLVVWVDPLDGTKEYTEGLLDNVTVLIGIA
  YEGKAIAGIINQPYYNYQAGPDAVLGRTIWGVLGLGAFGFQLKEAPAGKRIITTTRSHSNQLVTDCISAM
  NPDTVLRVGGAGNKIIQLIEGKASAYVFASPGCKKWDTCAPEVILHAVGGKLTDIHGNALQYNKEVKHMN
  SAGVLAALRNYEYYASHVPESVKNALIP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC011036
• ORF Size: 926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC011036, AAH11036
• RefSeq Size: 1165
• RefSeq ORF: 926
• Locus ID: 23827
• Gene Summary: Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Has 1000-fold lower activity towards inositol 1,4-bisphosphate (Ins(1,4)P2) and inositol 1,3,4-trisphosphate (Ins(1,3,4)P3), but does not hydrolyze Ins(1)P, Ins(3,4)P2, Ins(1,3,4,5)P4 or InsP6. [UniProtKB/Swiss-Prot Function]