Ggh (NM_010281) Mouse Tagged ORF Clone

CAT#: MG204547

Ggh (GFP-tagged) - Mouse gamma-glutamyl hydrolase (Ggh)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ggh
• Synonyms: gamma-GH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204547 representing NM_010281
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAACCTGGGCTACCTGCTGTGCTTGTTGGGTTTGCTACTATGCGGGCTTTCAAGTCCCGGGATGT
  CCAGACCCTACAACCATGGCTCCGAGAGGCCCATCATCGGAGTAGTAATGCAAGAATGTTTTGGGAAAAT
  GGCGAAGTTGGGAAACTACTACATTGCTGCATCCTATGTGAAGTATATAGAGTCTGCAGGTGCACGAGTT
  GTGCCCATAAGGCCTGATCTTAGTGATGCAGAGTATGAAGAACTTTTTAGATCTATTAATGGAGTTCTCC
  TTCCTGGAGGAGGTGCTAACCTAACAGATTCAGGTTATTCCCGAGTGGCCAAAATATTTTTCAGCAAGGC
  TCTAGAGAGCTTTGATAATGGAGGCCATTTTCCTGTGTGGGGAACATGCCTTGGATTTGAGGAGCTTTCT
  GTCCTGGTTAGTGGAGAGAACTTACTAACGAGTACAGACACTAAGTCAAAGAAATTGCCCTTGAACTTCA
  CTGAAGGTGCAAGGAAGAGCAGAATGTTCAAACATTTTCCTACTGAGTTATTGGATTCATTAGCACTAGA
  AAATCTGACCGCAAATTTTCACAAATGGAGCCTCTCTGTGAAGAATTTTACAGAAAATGAAAAGTTAAAG
  AAGTTTTTCAATATATTAACAACCAATACAGATGGCAAAACTGAGTTCATTTCATCCATGGAAGGATTTA
  AGTATCCAGTGTATGCTGTCCAGTGGCATCCTGAGAAAGCACCATTTGAGTGGAAGAATCTGGGCGGCAT
  TTCTCATGCACCAAATGCTGTGAAGACTTCATTCTACTTAGCAGAATTTTTAGTTTCTGAAGCTCGGAAG
  AACAGTCACCACTTTGAGAACGTGGTTAAGGAGACGGCATCCTTGATTTACAAGGTCTATCCCATTTATA
  CTGGAAATATTTCTTCATTTCAGCAAGCTTATATGTTTGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204547 representing NM_010281
  Red=Cloning site Green=Tags(s)
  
  MANLGYLLCLLGLLLCGLSSPGMSRPYNHGSERPIIGVVMQECFGKMAKLGNYYIAASYVKYIESAGARV
  VPIRPDLSDAEYEELFRSINGVLLPGGGANLTDSGYSRVAKIFFSKALESFDNGGHFPVWGTCLGFEELS
  VLVSGENLLTSTDTKSKKLPLNFTEGARKSRMFKHFPTELLDSLALENLTANFHKWSLSVKNFTENEKLK
  KFFNILTTNTDGKTEFISSMEGFKYPVYAVQWHPEKAPFEWKNLGGISHAPNAVKTSFYLAEFLVSEARK
  NSHHFENVVKETASLIYKVYPIYTGNISSFQQAYMFD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010281
• ORF Size: 951 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010281.2, NP_034411.2
• RefSeq Size: 1340
• RefSeq ORF: 954
• Locus ID: 14590
• Gene Summary: Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Acts as endopeptidase. Lysosomal enzyme is activated by sulfhydryl compounds. [UniProtKB/Swiss-Prot Function]