Ggh (NM_010281) Mouse Tagged ORF Clone

CAT#: MR204547

Ggh (Myc-DDK-tagged) - Mouse gamma-glutamyl hydrolase (Ggh)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ggh
• Synonyms: gamma-GH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204547 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAACCTGGGCTACCTGCTGTGCTTGTTGGGTTTGCTACTATGCGGGCTTTCAAGTCCCGGGATGT
  CCAGACCCTACAACCATGGCTCCGAGAGGCCCATCATCGGAGTAGTAATGCAAGAATGTTTTGGGAAAAT
  GGCGAAGTTGGGAAACTACTACATTGCTGCATCCTATGTGAAGTATATAGAGTCTGCAGGTGCACGAGTT
  GTGCCCATAAGGCCTGATCTTAGTGATGCAGAGTATGAAGAACTTTTTAGATCTATTAATGGAGTTCTCC
  TTCCTGGAGGAGGTGCTAACCTAACAGATTCAGGTTATTCCCGAGTGGCCAAAATATTTTTCAGCAAGGC
  TCTAGAGAGCTTTGATAATGGAGGCCATTTTCCTGTGTGGGGAACATGCCTTGGATTTGAGGAGCTTTCT
  GTCCTGGTTAGTGGAGAGAACTTACTAACGAGTACAGACACTAAGTCAAAGAAATTGCCCTTGAACTTCA
  CTGAAGGTGCAAGGAAGAGCAGAATGTTCAAACATTTTCCTACTGAGTTATTGGATTCATTAGCACTAGA
  AAATCTGACCGCAAATTTTCACAAATGGAGCCTCTCTGTGAAGAATTTTACAGAAAATGAAAAGTTAAAG
  AAGTTTTTCAATATATTAACAACCAATACAGATGGCAAAACTGAGTTCATTTCATCCATGGAAGGATTTA
  AGTATCCAGTGTATGCTGTCCAGTGGCATCCTGAGAAAGCACCATTTGAGTGGAAGAATCTGGGCGGCAT
  TTCTCATGCACCAAATGCTGTGAAGACTTCATTCTACTTAGCAGAATTTTTAGTTTCTGAAGCTCGGAAG
  AACAGTCACCACTTTGAGAACGTGGTTAAGGAGACGGCATCCTTGATTTACAAGGTCTATCCCATTTATA
  CTGGAAATATTTCTTCATTTCAGCAAGCTTATATGTTTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204547 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MANLGYLLCLLGLLLCGLSSPGMSRPYNHGSERPIIGVVMQECFGKMAKLGNYYIAASYVKYIESAGARV
  VPIRPDLSDAEYEELFRSINGVLLPGGGANLTDSGYSRVAKIFFSKALESFDNGGHFPVWGTCLGFEELS
  VLVSGENLLTSTDTKSKKLPLNFTEGARKSRMFKHFPTELLDSLALENLTANFHKWSLSVKNFTENEKLK
  KFFNILTTNTDGKTEFISSMEGFKYPVYAVQWHPEKAPFEWKNLGGISHAPNAVKTSFYLAEFLVSEARK
  NSHHFENVVKETASLIYKVYPIYTGNISSFQQAYMFD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010281
• ORF Size: 954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010281.2, NP_034411.2
• RefSeq Size: 1340 bp
• RefSeq ORF: 954 bp
• Locus ID: 14590
• MW: 35.4 kDa
• Gene Summary: Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Acts as endopeptidase. Lysosomal enzyme is activated by sulfhydryl compounds. [UniProtKB/Swiss-Prot Function]