Prkag1 (NM_016781) Mouse Tagged ORF Clone

CAT#: MG204858

Prkag1 (GFP-tagged) - Mouse protein kinase, AMP-activated, gamma 1 non-catalytic subunit (Prkag1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prkag1
• Synonyms: AA571379; BB036179; Prkaac
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204858 representing NM_016781
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCGGTTGCTGCAGAGAGCTCGCCAGCTCTAGAGAATGAACACTTTCAAGAGACCCCGGAATCAA
  ACAATAGTGTGTATACTTCCTTCATGAAGTCTCATCGCTGCTATGACCTAATTCCCACAAGTTCCAAGTT
  GGTGGTATTTGACACTTCGCTACAGGTAAAGAAAGCCTTTTTTGCCCTGGTGACCAATGGTGTTCGTGCC
  GCCCCTTTGTGGGACAGTAAGAAGCAGAGTTTTGTGGGCATGCTGACCATCACCGACTTCATCAACATTT
  TGCACCGATACTATAAGTCAGCCCTGGTGCAGATCTACGAACTGGAGGAGCACAAGATAGAGACGTGGAG
  AGAGGTGTACCTGCAGGACTCCTTTAAGCCACTTGTCTGCATCTCTCCAAATGCCAGCTTGTTTGATGCT
  GTCTCTTCATTAATTCGAAATAAGATCCACAGGCTCCCAGTTATCGACCCAGAGTCAGGCAACACCTTGT
  ACATCCTTACTCACAAGCGGATCCTCAAGTTCCTCAAGTTGTTTATCACCGAGTTCCCCAAGCCGGAATT
  CATGTCTAAGTCTCTCCAAGAGCTGCAGATTGGCACCTATGCCAATATTGCCATGGTCCGTACTACCACG
  CCTGTCTACGTGGCTCTGGGCATCTTTGTACAGCACCGAGTCTCCGCCTTACCTGTAGTGGATGAGAAAG
  GGCGTGTGGTGGACATCTACTCCAAGTTTGATGTGATCAATTTGGCAGCCGAAAAGACCTACAACAACCT
  AGATGTGTCTGTGACAAAAGCCCTGCAGCATCGGTCCCACTACTTTGAGGGTGTTCTCAAATGCTACCTG
  CATGAGACTCTGGAAACCATCATCAATAGGCTGGTGGAGGCAGAGGTTCACCGTCTGGTGGTGGTGGATG
  AACACGACGTGGTCAAGGGCATCGTTTCGCTGTCTGACATCTTACAGGCTCTGGTGCTCACGGGTGGAGA
  GAAGAAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204858 representing NM_016781
  Red=Cloning site Green=Tags(s)
  
  MESVAAESSPALENEHFQETPESNNSVYTSFMKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRA
  APLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDA
  VSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLQELQIGTYANIAMVRTTT
  PVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYL
  HETLETIINRLVEAEVHRLVVVDEHDVVKGIVSLSDILQALVLTGGEKKP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016781
• ORF Size: 990 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016781.2, NP_058061.2
• RefSeq Size: 1680
• RefSeq ORF: 993
• Locus ID: 19082