Ift74 (BC049156) Mouse Tagged ORF Clone

CAT#: MG204909

Ift74 (GFP-tagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (cDNA clone MGC:56922 IMAGE:6313441)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ift74
• Synonyms: Cmg1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204909 representing BC049156
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGTAATCACAAATCTTCAGCCCCTCGCCCTATTTCTCGGGGTGGAATAGGACTAACAGGAAGGC
  CTCCTTCTGGAATAAGACCTCCATCTGGCAATGTTCGAGTGGCAACTGCAATGCCACCAACAACAGCAAG
  ACCAGGTTCTCGTGGTGGTCCCTTAGGGACTGGTGGAGTTTTGTCATCTCAAATCAAAGTTGCTGATCGT
  CCTGTGACCCAACAAGGTTTGAGTGGAATGAAGACTGGCATGAAAGGTCCCCAGAGGCAAATTTTAGACA
  AATCTTACTATCTTGGACTTCTTAGGAGCAAAATAAGTGAACTTACAACTGAAATTAATAAGCTTCAGAA
  AGAAATAGAAATGTACAACCAAGAAAATTCAGTGTATTTGTCATATGAAAAGAGGGCAGAGACATTAGCT
  GTTGAAATTAAAGACTTTCAAGGACAACTAGCAGACTACAACATGTTGGTAGATAAACTTAATACCAATA
  CTGAAATGGAAGAAGTAATGAGTGATTACAACATGCTTAAAGCTCAAAATGACAGAGAAACACAAAGTAT
  GGATGTCATATTTACTGAAAGACAAGCGAAGGAAAAACAAATCAGAAGTGTAGAAGAAGAAGTTGAACAA
  GAAAAGCAAGCAGCAGATGGCATTATCAAAAATATGTCTCCTGAAAAACAAGTCAAGTACATAGAAATGA
  AAACCACAAATGAGAAACTGTTGCAGGAATTAGATACACTTCAGCAACAGCTAGATTCTCTGAACATGAA
  AAAGGAGAGCCTAGAAACTGAAATAGCACACTCCCAAGTAAAACAAGAGGCTGTATTGCTGTATGAAAAA
  CTTTATGAGTTAGAGTCCCATCGAGATCAAATGATTGCAGAAGACAAAAGCATGGGATCGCCAATGGAGG
  AAAGGGAGAGATTGTTTAAGCAGGTTAAAGAAGATAATCAGGAAATAGCCAGCATGGAGAGACAGTTAAC
  AGATATAAAAGAAAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204909 representing BC049156
  Red=Cloning site Green=Tags(s)
  
  MASNHKSSAPRPISRGGIGLTGRPPSGIRPPSGNVRVATAMPPTTARPGSRGGPLGTGGVLSSQIKVADR
  PVTQQGLSGMKTGMKGPQRQILDKSYYLGLLRSKISELTTEINKLQKEIEMYNQENSVYLSYEKRAETLA
  VEIKDFQGQLADYNMLVDKLNTNTEMEEVMSDYNMLKAQNDRETQSMDVIFTERQAKEKQIRSVEEEVEQ
  EKQAADGIIKNMSPEKQVKYIEMKTTNEKLLQELDTLQQQLDSLNMKKESLETEIAHSQVKQEAVLLYEK
  LYELESHRDQMIAEDKSMGSPMEERERLFKQVKEDNQEIASMERQLTDIKEK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC049156
• ORF Size: 998 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC049156
• RefSeq Size: 1659
• RefSeq ORF: 998
• Locus ID: 67694