Gja4 (NM_008120) Mouse Tagged ORF Clone

CAT#: MG204914

Gja4 (GFP-tagged) - Mouse gap junction membrane channel protein alpha 4 (Gja4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gja4
• Synonyms: AU020209; AW558810; Cnx37; Cx37; Gja-4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204914 representing NM_008120
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACTGGGGCTTCCTGGAGAAGTTGCTAGACCAGGTCCAGGAACACTCGACCGTGGTGGGCAAGA
  TCTGGTTAACGGTGCTCTTCATCTTCCGCATCCTCATCCTGGGGCTGGCTGGCGAGTCGGTGTGGGGCGA
  CGAGCAGTCTGATTTTGAGTGTAACACAGCCCAGCCGGGCTGCACCAACGTCTGCTATGACCAGGCCTTC
  CCCATCTCCCACATCCGATACTGGGTGCTGCAGTTCCTCTTCGTCAGCACACCCACCCTGATCTACCTGG
  GCCATGTCATCTACCTGTCTCGGCGGGAAGAGCGGTTGCGGCAGAAAGAGGGAGAGCTCCGGGCGCTGCC
  ATCCAAGGACCTACATGTAGAGCGGGCACTGGCTGCCATCGAACATCAGATGGCCAAGATCTCGGTGGCA
  GAGGACGGTCGTCTTCGGATTCGTGGGGCGCTCATGGGTACCTATGTGGTCAGCGTGCTGTGTAAGAGTG
  TGCTGGAGGCAGGCTTCCTCTATGGCCAGTGGCGCCTCTATGGCTGGACCATGGAGCCGGTGTTTGTGTG
  CCAGCGTGCACCCTGCCCCCACATCGTGGACTGCTATGTCTCTCGACCCACTGAGAAGACTATCTTCATC
  ATCTTCATGCTGGTGGTAGGAGTCATCTCCCTGGTGCTCAACCTGCTGGAGCTGGTTCACCTGCTGTGTC
  GGTGTGTCAGCCGGGAGATAAAGGCACGAAGGGACCAGGACGCCCGCCCGGCCCAGGGCAGTGCCTCAGA
  CCCTTACCCTGAACAGGTTTTCTTCTACCTCCCCATGGGCGAGGGACCCTCTTCCCCACCGTGTCCCACC
  TACAACGGGCTCTCATCCACTGAGCAGAACTGGGCCAACTTGACCACAGAGGAGAGACTGACCTCTTCCA
  GACCTCCCCCATTTGTAAACACAGCTCCCCAGGGTGGCCGAAAGTCCCCTAGCCGCCCCAACAGCTCTGC
  ATCCAAGAAGCAGTATGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204914 representing NM_008120
  Red=Cloning site Green=Tags(s)
  
  MGDWGFLEKLLDQVQEHSTVVGKIWLTVLFIFRILILGLAGESVWGDEQSDFECNTAQPGCTNVCYDQAF
  PISHIRYWVLQFLFVSTPTLIYLGHVIYLSRREERLRQKEGELRALPSKDLHVERALAAIEHQMAKISVA
  EDGRLRIRGALMGTYVVSVLCKSVLEAGFLYGQWRLYGWTMEPVFVCQRAPCPHIVDCYVSRPTEKTIFI
  IFMLVVGVISLVLNLLELVHLLCRCVSREIKARRDQDARPAQGSASDPYPEQVFFYLPMGEGPSSPPCPT
  YNGLSSTEQNWANLTTEERLTSSRPPPFVNTAPQGGRKSPSRPNSSASKKQYV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008120
• ORF Size: 999 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008120.2, NP_032146.1
• RefSeq Size: 1633
• RefSeq ORF: 1002
• Locus ID: 14612
• Gene Summary: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. [UniProtKB/Swiss-Prot Function]