Kcnk1 (NM_008430) Mouse Tagged ORF Clone

CAT#: MG204992

Kcnk1 (GFP-tagged) - Mouse potassium channel, subfamily K, member 1 (Kcnk1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kcnk1
• Synonyms: AI788889; TWIK-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204992 representing NM_008430
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCAGTCCCTGGCCGGCAGCTCGTGCGTGCGCCTGGTGGAGCGGCACCGTTCGGCCTGGTGCTTCG
  GCTTCCTGGTGCTGGGCTACCTGCTCTACCTGGTGTTCGGCGCCGTGGTCTTCTCGTCCGTGGAGCTGCC
  TTATGAGGACCTGCTGCGCCAGGAGCTGCGCAAGCTGAAGCGGCGCTTCCTGGAGGAGCACGAGTGCCTG
  TCGGAGCCGCAGCTGGAGCAGTTCCTGGGCCGCGTGCTGGAGGCCAGCAATTATGGAGTGTCGGTGCTCA
  GCAACGCCTCGGGAAATTGGAATTGGGACTTCACCTCGGCGCTCTTCTTCGCCAGCACCGTGCTCTCCAC
  CACAGGCTATGGCCACACGGTGCCCCTGTCAGATGGGGGCAAAGCCTTCTGCATCATCTACTCTGTCATC
  GGCATCCCGTTCACCCTCCTCTTCCTGACGGCCGTGGTCCAGCGTGTCACCGTGCATGTCACCCGCAGAC
  CAGTCCTCTACTTCCACATACGCTGGGGCTTCTCCAAGCAGGTGGTGGCCATTGTCCATGCCGTTCTGCT
  GGGATTTGTCACCGTTTCCTGCTTCTTCTTCATCCCAGCCGCCGTGTTCTCTGTGCTGGAGGATGACTGG
  AACTTCCTCGAGTCTTTTTACTTCTGTTTCATCTCCCTGAGCACCATCGGCCTGGGGGACTATGTTCCAG
  GGGAAGGCTACAACCAGAAGTTCCGAGAGCTGTACAAGATCGGAATCACGTGTTACCTGCTCCTCGGACT
  CATCGCCATGCTGGTTGTCCTGGAGACCTTCTGTGAACTCCACGAGCTGAAGAAGTTCAGGAAGATGTTC
  TACGTGAAGAAAGACAAGGACGAAGACCTGGTTCACATCATGGAGCACGACCAGCTGTCCTTCTCCTCCG
  TCACTGAGCAGGTGGCTGGCCTGAAGGAGGAGCAGAAGCAAAGTGAGCCTTTTGTGGCCTCCCAGTCCCC
  ACCCTATGAGGATGGCTCTGCAGACCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204992 representing NM_008430
  Red=Cloning site Green=Tags(s)
  
  MLQSLAGSSCVRLVERHRSAWCFGFLVLGYLLYLVFGAVVFSSVELPYEDLLRQELRKLKRRFLEEHECL
  SEPQLEQFLGRVLEASNYGVSVLSNASGNWNWDFTSALFFASTVLSTTGYGHTVPLSDGGKAFCIIYSVI
  GIPFTLLFLTAVVQRVTVHVTRRPVLYFHIRWGFSKQVVAIVHAVLLGFVTVSCFFFIPAAVFSVLEDDW
  NFLESFYFCFISLSTIGLGDYVPGEGYNQKFRELYKIGITCYLLLGLIAMLVVLETFCELHELKKFRKMF
  YVKKDKDEDLVHIMEHDQLSFSSVTEQVAGLKEEQKQSEPFVASQSPPYEDGSADH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008430
• ORF Size: 1008 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008430.1
• RefSeq Size: 2306
• RefSeq ORF: 1011
• Locus ID: 16525