Sult2b1 (NM_017465) Mouse Tagged ORF Clone

CAT#: MG205040

Sult2b1 (GFP-tagged) - Mouse sulfotransferase family, cytosolic, 2B, member 1 (Sult2b1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sult2b1
• Synonyms: AI326997; BB173635; ST2B1; SULT2B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205040 representing NM_017465
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGGCCGCAGCCCCGCGCCCTGTGGAGCTCGTCTGAGAAAAATGTTTCCGAAATGAGCTGGAATT
  TTGGAGGTGAATACTTCAGATACAAAGGTATCCCTTTTCCTGTCGGCATGTACTCACCGGAGAGCCTCAG
  TCTGGCTGAGAACACTAGCAACGTGCGGGACGACGACATCTTCATTGTCACCTACCCCAAATCAGGTACC
  AACTGGATGATTGAGATCGTCTGCTTAATCCTGAAAGATGGGGATCCCTCGTGGATCCGATCGGAGCCCA
  TCTGGCAACGTGCGCCCTGGTGCGAGACCATCATAAGCGCCTTCAATGTCTTAGACCGGCCCAGCCCCCG
  CATTATGAGCTCTCACCTCCCTATTGAACTCTTCACGAAGGCATTCTTCAGCTCCAAGGCTAAGGTGATT
  TACGTGGGCCGGAACCCCCGCGATGTCGTGGTCTCCCTCTATTATTATTCTAAGATTGCTGGGCAATTAA
  AGGACCCTGGTACACCCGACCAGTTCCTTCAAAATTTCCTCAAAGGAGAAGTGCAGTTTGGCTCCTGGTT
  TGACCACATCAAGGGCTGGATCCGGATGCAGAACCAAGAGAACTTCCTGTTTATCACCTATGAGGAGCTG
  CAGCAGGACCTGCGAGGCTCCGTGCAACGCATCTGTGAGTTCCTGGGCCGGCCACTGGGTGAAGAGGCCC
  TGAGCTCTGTGGTGGCCCACTCAGCCTTTGCTGCCATGAAGGCCAATACCATGTCCAACTACTCGCTGCT
  GCCGGCCAGCCTGCTGGACCACCGCCAGGGGGAGTTCCTGCGCAAAGGGATCAGTGGCGACTGGAAGAAC
  CACTTCACTGTGGCCCAGAGTGAGGCTTTTGACAGTGTTTACCGAGAGCAAATGCACGGGGTGCAGAGGT
  TCCCCTGGGACACGTCTGAAGAGGATAGCAGCCCTGATGGCCAGCCTGACCCTGAGCCCAGCCCCAGCCC
  AGCTTCTGATGACCCCAACCCAGGATCCTCACAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205040 representing NM_017465
  Red=Cloning site Green=Tags(s)
  
  MDGPQPRALWSSSEKNVSEMSWNFGGEYFRYKGIPFPVGMYSPESLSLAENTSNVRDDDIFIVTYPKSGT
  NWMIEIVCLILKDGDPSWIRSEPIWQRAPWCETIISAFNVLDRPSPRIMSSHLPIELFTKAFFSSKAKVI
  YVGRNPRDVVVSLYYYSKIAGQLKDPGTPDQFLQNFLKGEVQFGSWFDHIKGWIRMQNQENFLFITYEEL
  QQDLRGSVQRICEFLGRPLGEEALSSVVAHSAFAAMKANTMSNYSLLPASLLDHRQGEFLRKGISGDWKN
  HFTVAQSEAFDSVYREQMHGVQRFPWDTSEEDSSPDGQPDPEPSPSPASDDPNPGSSQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017465
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017465.1, NP_059493.1
• RefSeq Size: 1127
• RefSeq ORF: 1017
• Locus ID: 54200
• Gene Summary: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids such as dehydroepiandrosterone. Isoform 1 is required for production of cholesterol sulfate essential for normal skin development whereas isoform 2 produces pregnenolone sulfate, an essential neurosteroid during development of the central nervous system. [UniProtKB/Swiss-Prot Function]