Rfc5 (NM_028128) Mouse Tagged ORF Clone

CAT#: MG205065

Rfc5 (GFP-tagged) - Mouse replication factor C (activator 1) 5 (Rfc5)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rfc5
• Synonyms: 36.5kDa; 36kDa; 2610020K06Rik; 2610209F07Rik; Recc5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205065 representing NM_028128
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCGGCCGCGCCTTCGCAGCAGCGGCCCGCGGCGGCCCGGGCCCGGAACCTGCCCTGGGTTGAGA
  AGTACCGGCCACAGACCCTGGCCGATCTCATTTCTCACCAGGACATTCTGAGTACCATTCAGAAGTTCAT
  CAGTGAAGACCGCCTGCCACACCTACTTCTCTATGGCCCTCCAGGGACAGGAAAGACATCCACCATCCTG
  GCCTGTGCCAAGCAACTGTACAAAGATAAAGAATTTGGCTCCATGGTCTTGGAGCTGAATGCTTCTGACG
  ACCGAGGGATCGATATTGTTCGGGGGCCAATCCTCAGCTTTGCCAGCACAAGGACAATCTTCAAGAAAGG
  GTTTAAGCTTGTGATCCTGGACGAAGCCGACGCCATGACTCAGGATGCCCAGAACGCCTTGAGACGAGTG
  ATTGAGAAGTTCACAGAAAACACCAGGTTCTGCCTCATCTGTAACTACCTGTCCAAGATCATCCCTGCCT
  TGCAGTCTCGGTGCACGAGGTTCCGATTTGGCCCTCTGACACCCGAGCTCATGGTCCCTCGACTGGAACA
  TGTAGTACAAGAAGAGAATGTGGATATAAGTGAAGATGGAATGAAGGCCCTTGTCACTCTGTCCAGTGGG
  GACATGCGAAGGGCTTTGAACATCCTTCAGAGTACCAATATGGCCTTTGGGAAGGTGACAGAGGAGACTG
  TCTACACCTGCACCGGACACCCACTCAAGACGGACATTGCCAATATACTGGACTGGATGCTCAATCAGGA
  CTTCACCACTGCCTACAAAAACATCATGGAGCTGAAGACTCTGAAGGGCTTGGCGCTGCATGACATCCTG
  ACAGAGGTTCACTTGTTTGTACACAGAGTTGACTTTCCATCTTCAGTTCGGATACACTTATTGACCAAAA
  TGGCAGACATCGAGTACAGACTTTCTGTTGGCACCAGTGAGAAGATACAGCTGAGCTCCCTCATCGCCGC
  TTTTCAAGTCACCAGAGACCTGATAGTTGCAGAGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205065 representing NM_028128
  Red=Cloning site Green=Tags(s)
  
  MTAAAPSQQRPAAARARNLPWVEKYRPQTLADLISHQDILSTIQKFISEDRLPHLLLYGPPGTGKTSTIL
  ACAKQLYKDKEFGSMVLELNASDDRGIDIVRGPILSFASTRTIFKKGFKLVILDEADAMTQDAQNALRRV
  IEKFTENTRFCLICNYLSKIIPALQSRCTRFRFGPLTPELMVPRLEHVVQEENVDISEDGMKALVTLSSG
  DMRRALNILQSTNMAFGKVTEETVYTCTGHPLKTDIANILDWMLNQDFTTAYKNIMELKTLKGLALHDIL
  TEVHLFVHRVDFPSSVRIHLLTKMADIEYRLSVGTSEKIQLSSLIAAFQVTRDLIVAEA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028128
• ORF Size: 1017 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_028128.1, NP_082404.1
• RefSeq Size: 1750
• RefSeq ORF: 1020
• Locus ID: 72151
• Gene Summary: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. [UniProtKB/Swiss-Prot Function]