Dnajb1 (NM_018808) Mouse Tagged ORF Clone

CAT#: MG205083

Dnajb1 (GFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 1 (Dnajb1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dnajb1
• Synonyms: 0610007I11Rik; DjB1; Hdj1; Hsp40; HSPF1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205083 representing NM_018808
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGGACTACTATCAGACGCTGGGCCTGGCCCGCGGCGCGTCCGACGACGAGATCAAGCGAGCCT
  ACCGCCGCCAGGCGCTGCGCTACCACCCGGACAAGAACAAGGAGCCCGGCGCTGAGGAGAAGTTCAAGGA
  GATCGCCGAGGCCTACGACGTGCTCAGCGACCCGCGCAAGCGCGAGATTTTCGACCGCTATGGAGAGGAA
  GGCCTGAAGGGTGGTAGCCCCAGTGGAGGAAGCAGTGGTGGTGCTAATGGTACCTCTTTCAGCTACACAT
  TCCACGGAGACCCCCATGCCATGTTTGCTGAGTTCTTCGGTGGCAGAAACCCCTTTGATACCTTTTTTGG
  GCAGCGCAACGGGGAAGAAGGCATGGACATTGATGACACATTCTCTAGCTTTCCAATGGGTATGGGTGGC
  TTCACCAACATGAACTTTGGACGTTCCCGCCCTTCTCAAGAGCCCACCCGGAAGAAGCAAGATCCTCCAG
  TCACCCATGACCTTCGGGTCTCCCTTGAAGAGATCTACAGCGGCTGTACCAAGAAGATGAAAATCTCCCA
  CAAGCGGCTGAACCCTGATGGAAAGAGCATTCGAAATGAAGATAAGATCCTGACCATCGAAGTGAAGAGG
  GGCTGGAAAGAAGGGACCAAAATCACCTTTCCCAAGGAAGGGGACCAGACCTCGAACAACATTCCAGCAG
  ACATCGTCTTTGTTTTAAAGGACAAGCCACACAATATCTTCAAGAGAGATGGTTCTGATGTCATCTATCC
  AGCCAGGATTAGCCTTCGGGAGGCTCTCTGTGGTTGCACTGTGAATGTCCCTACTCTGGACGGCAGGACC
  ATCCCTGTTGTATTCAAAGATGTCATCAGGCCTGGTATGCGGCGGAAAGTCCCTGGAGAAGGCCTCCCTC
  TCCCCAAAACACCTGAGAAACGTGGAGACCTTGTTATCGAGTTTGAAGTGATCTTCCCGGAAAGGATTCC
  CGTCTCATCCAGAACCATCCTGGAGCAGGTTCTTCCCATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205083 representing NM_018808
  Red=Cloning site Green=Tags(s)
  
  MGKDYYQTLGLARGASDDEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEE
  GLKGGSPSGGSSGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDTFSSFPMGMGG
  FTNMNFGRSRPSQEPTRKKQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKR
  GWKEGTKITFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRT
  IPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLVIEFEVIFPERIPVSSRTILEQVLPI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018808
• ORF Size: 1020 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018808.1, NP_061278.1
• RefSeq Size: 2279
• RefSeq ORF: 1023
• Locus ID: 81489
• Gene Summary: This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. The encoded protein may also inhibit apoptosis. Peritoneal macrophages derived from homozygous knockout mice for this gene exhibit impaired heat tolerance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]