Rfc3 (NM_027009) Mouse Tagged ORF Clone

CAT#: MG205432

Rfc3 (GFP-tagged) - Mouse replication factor C (activator 1) 3 (Rfc3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rfc3
• Synonyms: 38kDa; 2810416I22Rik; AU022547; Recc3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205432 representing NM_027009
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCTCTGGGTGGACAAGTACCGGCCGAGCTCCCTTGCCCGGCTGGACTATCATAAGGAACAGGCAG
  CACAGCTGCGCAACCTGGTGCAGTGTGGAGACTTTCCTCACCTCTTAGTATATGGACCATCAGGTGCTGG
  AAAAAAGACAAGAATTATGTGTATTCTACGTGAACTTTATGGTATCGGGGTGGAAAAACTGAGAATTGAG
  CATCAGACTATCACAACCCCGTCTAAAAAAAAAATTGAAATCAGCACCATTGCAAGCAACTACCACCTTG
  AAGTTAATCCCAGTGATGCGGGGAACAGCGACCGAGTTGTAATTCAGGAGATGCTGAAGACAGTGGCACA
  GTCACAACAACTTGAAACCAGCTCACAAAGAGACTTCAAAGTGGTATTATTGACAGAGGTGGACAAACTC
  ACAAAGGATGCTCAGCATGCCTTACGCAGAACTATGGAGAAATACATGTCTACCTGCAGGCTCATCTTGT
  GCTGCAATTCCACATCTAAGGTGATACCCCCGATCCGAAGCAGGTGCCTGGCAGTTCGTGTGCCTGCTCC
  CAGCATCGAAGATATTTGCAGTGTACTATCCACTGTCTGCAGGAAGGAAGGTCTGGCTCTCCCTTCGACA
  TTGGCCCGTAGGCTGGCCGAGAAGTCATGCCGAAACCTCAGGAAAGCCTTACTTATGTGTGAGGCCTGCA
  GAGTGCAGCAATACCCTTTTACGGAAGACCAAGAAATCCCAGAGACAGACTGGGAGGTGTATCTGAGGGA
  GACCGCAAATGCTATTGTCAGTCAGCAGACGCCACAGAGGCTCCTTGAAGTCCGTGGAAGGCTCTATGAG
  CTTCTAACTCATTGTATTCCTCCTGAGATAATAATGAAGGGCCTCCTCTCAGAACTCCTCCATAACTGTG
  ATGGGCAGCTGAAAGGGGAAGTGGCACAGATGGCAGCCTACTATGAGCACCGACTACAGCTGGGCAGCAA
  AGCCATTTATCATTTGGAAGCATTTGTGGCGAAGTTTATGGCACTTTATAAGAAGTTCATGGAGGATGGA
  CTGGAAGGCATGATGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205432 representing NM_027009
  Red=Cloning site Green=Tags(s)
  
  MSLWVDKYRPSSLARLDYHKEQAAQLRNLVQCGDFPHLLVYGPSGAGKKTRIMCILRELYGIGVEKLRIE
  HQTITTPSKKKIEISTIASNYHLEVNPSDAGNSDRVVIQEMLKTVAQSQQLETSSQRDFKVVLLTEVDKL
  TKDAQHALRRTMEKYMSTCRLILCCNSTSKVIPPIRSRCLAVRVPAPSIEDICSVLSTVCRKEGLALPST
  LARRLAEKSCRNLRKALLMCEACRVQQYPFTEDQEIPETDWEVYLRETANAIVSQQTPQRLLEVRGRLYE
  LLTHCIPPEIIMKGLLSELLHNCDGQLKGEVAQMAAYYEHRLQLGSKAIYHLEAFVAKFMALYKKFMEDG
  LEGMMF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027009
• ORF Size: 1068 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_027009.1, NP_081285.1
• RefSeq Size: 1217
• RefSeq ORF: 1071
• Locus ID: 69263
• Gene Summary: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. [UniProtKB/Swiss-Prot Function]