Hmbs (BC003861) Mouse Tagged ORF Clone

CAT#: MG205538

Hmbs (GFP-tagged) - Mouse hydroxymethylbilane synthase (cDNA clone MGC:6604 IMAGE:3487642)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hmbs
• Synonyms: PBGD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205538 representing BC003861
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGTAACGGCGGCGCGGCCACAACCGCGGAAGAAAACGGCTCAAAGATGAGGGTGATTCGAGTGG
  GCACCCGTAAGAGCCAGCTGGCTCGCATACAGACCGACACTGTGGTGGCGATGCTGAAAGCCTTGTACCC
  TGGCATACAGTTTGAAATCATTGCTATGTCCACCACGGGAGACAAGATTCTTGATACTGCACTCTCTAAG
  ATTGGAGAGAAGAGCCTGTTTACCAAGGAGCTAGAAAACGCCCTGGAAAAAAACGAAGTGGACCTGGTTG
  TTCACTCCCTGAAGGATGTGCCTACCATACTACCTCCTGGCTTTACTATTGGAGCCATCTGCAAACGGGA
  AAACCCTTGTGATGCTGTTGTCTTTCACCCAAAGTTTATTGGAAAGACCCTGGAAACCTTGCCAGAGAAA
  AGTGCCGTGGGAACCAGCTCTCTGAGGAGAGTGGCTCAGCTACAGAGAAAGTTCCCCAACCTGGAATTCA
  AGAGTATTCGGGGAAACCTCAACACCCGCCTTCGGAAGCTGGATGAGCTGCAGGAATTCAGTGCCATTGT
  CCTGGCTGTGGCTGGCCTACAGCGCATGGGCTGGCAGAACCGGGTGGGCCAGATTTTGCACCCAGAGGAA
  TGCATGTATGCTGTGGGTCAGGGGGCCCTAGCCGTGGAAGTCCGAGCCAAGGACCAGGATATCTTGGACC
  TAGTGAGTGTGTTGCACGATCCTGAAACTCTGCTTCGCTGCATTGCTGAAAGGGCTTTTCTGAGGCACCT
  GGAAGGAGGCTGCAGCGTGCCCGTAGCAGTGCATACAGTGATGAAAGATGGGCAACTGTACCTGACTGGT
  GGAGTATGGAGTCTAGATGGCTCAGATAGCATGCAAGAGACTATGCAGGCCACCATCCAGGTCCCTGTTC
  AGCAAGAAGATGGTCCAGAAGATGACCCACAACTGGTTGGAATCACTGCCCGGAACATTCCAAGAGGAGC
  CCAGCTAGCTGCTGAGAACCTGGGCATCAGCCTGGCCAGCTTGCTGCTCAACAAAGGAGCCAAGAACATC
  CTGGATGTTGCACGGCAGCTTAATGATGTGCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205538 representing BC003861
  Red=Cloning site Green=Tags(s)
  
  MSGNGGAATTAEENGSKMRVIRVGTRKSQLARIQTDTVVAMLKALYPGIQFEIIAMSTTGDKILDTALSK
  IGEKSLFTKELENALEKNEVDLVVHSLKDVPTILPPGFTIGAICKRENPCDAVVFHPKFIGKTLETLPEK
  SAVGTSSLRRVAQLQRKFPNLEFKSIRGNLNTRLRKLDELQEFSAIVLAVAGLQRMGWQNRVGQILHPEE
  CMYAVGQGALAVEVRAKDQDILDLVSVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTVMKDGQLYLTG
  GVWSLDGSDSMQETMQATIQVPVQQEDGPEDDPQLVGITARNIPRGAQLAAENLGISLASLLLNKGAKNI
  LDVARQLNDVR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC003861
• ORF Size: 1085 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC003861, AAH03861
• RefSeq Size: 1571
• RefSeq ORF: 1085
• Locus ID: 15288
• Gene Summary: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps. [UniProtKB/Swiss-Prot Function]