Plod1 (BC010268) Mouse Tagged ORF Clone

CAT#: MG205599

Plod1 (GFP-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (cDNA clone MGC:8124 IMAGE:3589182)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Plod1
• Synonyms: Plod, LH1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205599 representing BC010268
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCAGACCTGTGCCGTCAGGACCAAACTTGCACCTACTACTTCAGTGTGGATGCTGATGTGGCTC
  TGACGGAGCCGAACAGCCTAAGGCTCCTGATTGAACAAAACAAGAATGTCATTGCGCCACTCATGACCCG
  CCACGGGAGGCTCTGGTCCAACTTTTGGGGGGGTCTGAGTGCAGATGGGTACTACGCCCGCTCAGAGGAC
  TACGTGGACATTGTGCAGGGCCGTCGTGTTGGCGTCTGGAACGTGCCTTACATCTCAAACATCTACCTGA
  TCAAGGGCAGTGCCCTGCGGGCTGAACTGCAGAACGTAGACCTTTTCCACTACAGCAAGCTGGATTCCGA
  CATGAGTTTCTGCGCCAATGTCCGACAGCAGGAGGTGTTCATGTTCCTGACCAACCGGCACACCTTTGGC
  CACCTGCTTTCCCTGGATAACTACCAGACCACCCACCTACATAATGATCTCTGGGAGGTGTTCAGCAACC
  CTGAGGACTGGAAAGAAAAGTACATCCATGAGAATTACACCAAGGCCCTGGCGGGGAAGCTGGTGGAGAC
  GCCTTGTCCGGATGTCTACTGGTTCCCCATCTTCACGGAGGCGGCCTGTGATGAGCTGGTGGAGGAGATG
  GAACACTATGGCCAGTGGTCTCTGGGTGATAATAAGGACAACCGGATCCAGGGTGGCTACGAAAACGTGC
  CCACTATCGACATCCATATGAACCAGATCACCTTCGAGCGGGAGTGGCACAAGTTCCTGGTGGAGTACAT
  CGCCCCCATGACAGAGAAGCTGTACCCTGGCTACTACACTAGGGCCCAGTTTGATCTAGCCTTTGTCGTC
  CGCTATAAGCCTGATGAGCAGCCTTCCTTGATGCCCCACCATGACGCCTCTACCTTCACCGTCAACATAG
  CCCTGAACAGGGTTGGGGAAGATTATGAGGGCGGAGGTTGCCGATTTCTGCGCTACAACTGCTCCGTGAG
  GGCACCAAGGAAGGGCTGGGCCCTCCTGCACCCCGGGCGGCTCACACACTATCATGAGGGGCTTCCTACT
  ACCAAGGGCACGCGCTACATTGCTGTGTCTTTCGTCGATCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205599 representing BC010268
  Red=Cloning site Green=Tags(s)
  
  MGADLCRQDQTCTYYFSVDADVALTEPNSLRLLIEQNKNVIAPLMTRHGRLWSNFWGGLSADGYYARSED
  YVDIVQGRRVGVWNVPYISNIYLIKGSALRAELQNVDLFHYSKLDSDMSFCANVRQQEVFMFLTNRHTFG
  HLLSLDNYQTTHLHNDLWEVFSNPEDWKEKYIHENYTKALAGKLVETPCPDVYWFPIFTEAACDELVEEM
  EHYGQWSLGDNKDNRIQGGYENVPTIDIHMNQITFEREWHKFLVEYIAPMTEKLYPGYYTRAQFDLAFVV
  RYKPDEQPSLMPHHDASTFTVNIALNRVGEDYEGGGCRFLRYNCSVRAPRKGWALLHPGRLTHYHEGLPT
  TKGTRYIAVSFVDP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC010268
• ORF Size: 1094 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC010268, AAH10268
• RefSeq Size: 1903
• RefSeq ORF: 1094
• Locus ID: 18822
• Gene Summary: Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. [UniProtKB/Swiss-Prot Function]