Dcx (BC062974) Mouse Tagged ORF Clone

CAT#: MG205614

  • TrueORF®

Dcx (GFP-tagged) - Mouse doublecortin (cDNA clone MGC:61189 IMAGE:6825750)


Reconstitution Protocol

USD 330.00

3 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Dcx
Synonyms Dbct
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG205614 representing BC062974
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACTTGATTTTGGACATTTTGACGAACGAGACAAAGCATCTAGAAATATGAGAGGGTCACGGATGA
ATGGACTTCCAAGTCCCACTCATAGTGCCCACTGTAGCTTCTACAGAACCAGAACCTTGCAGGCATTAAG
TAATGAGAAGAAGGCCAAGAAGGTACGTTTCTACCGCAATGGGGACCGTTACTTCAAGGGGATTGTGTAC
GCTGTTTCTTCTGACCGTTTTCGTAGTTTTGATGCGTTGCTGGCTGACCTGACCCGATCCTTGTCTGACA
ACATTAACCTGCCTCAGGGAGTGCGCTACATTTATACCATTGACGGATCCAGGAAGATTGGAAGCATGGA
TGAACTGGAAGAAGGGGAAAGCTATGTCTGCTCCTCAGACAACTTCTTTAAAAAGGTTGAGTACACCAAG
AATGTCAACCCCAACTGGTCTGTCAACGTAAAGACATCTGCCAACATGAAAGCCCCCCAGTCCTTGGCTA
GCAGCAACAGTGCTCAAGCCAGAGAGAACAAGGACTTTGTGCGCCCCAAACTTGTGACCATCATTCGCAG
CGGGGTGAAGCCACGGAAGGCTGTGCGCGTGCTTCTCAACAAGAAAACAGCCCACTCTTTCGAGCAGGTC
CTGACTGACATCACAGAAGCGATCAAACTGGAAACCGGAGTTGTCAAAAAACTCTACACCCTTGATGGAA
AGCAGGTCACCTGTCTCCATGATTTCTTTGGTGATGATGATGTGTTCATTGCTTGTGGTCCTGAAAAATT
CCGCTATGCTCAAGATGATTTCTCCTTGGATGAGAATGAATGCAGAGTCATGAAAGGGAATCCATCTGCC
GCAGCTGGCCCAAAGGCTTCCCCAACACCTCAAAAGACATCTGCTAAAAGCCCAGGCCCAATGCGCCGCA
GCAAGTCTCCAGCTGACTCAGGTAACGACCAAGACGCAAATGGAACTTCCAGCAGTCAGCTCTCAACACC
TAAGTCAAAGCAGTCTCCTATCTCTACACCCACAAGCCCTGGAAGTCTGCGGAAGCACAAGGACCTGTAC
CTGCCGCTGTCATTGGATGACTCTGATTCACTTGGCGATTCCATG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG205614 representing BC062974
Red=Cloning site Green=Tags(s)

MELDFGHFDERDKASRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVY
AVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTK
NVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQV
LTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSA
AAGPKASPTPQKTSAKSPGPMRRSKSPADSGNDQDANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLY
LPLSLDDSDSLGDSM

TRTRRLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC062974
ORF Size 1097 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq BC062974, AAH62974
RefSeq Size 4102
RefSeq ORF 1097
Locus ID 13193
Gene Summary This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase. Studies in knockout mice lacking this gene and the LIS1 gene suggest that the molecular interaction of these two genes is important in both in neuronal migration and neurogenesis, and there is a cortical role of this gene in nuclear translocation and positioning of the mitotic spindle in radial glial mitotic division. Multiple transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]

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