Lefty2 (NM_177099) Mouse Tagged ORF Clone

CAT#: MG205670

Lefty2 (GFP-tagged) - Mouse Left-right determination factor 2 (Lefty2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lefty2
• Synonyms: 6030463A22Rik; Ebaf; Lefta; Leftb; lefty-2; lefty-B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205670 representing NM_177099
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTCCCTGTGGCTTTGCTGGGCACTCTGGGTACTGCCCCTGGCTGGCCCTGGGGCAGCGATGACCG
  AGGAACAGGTCCTGAGCAGTCTACTGCAGCAGCTGCAGCTCAGCCAGGCCCCCACCCTGGACAGCGCGGA
  TGTGGAGGAGATGGCCATCCCTACCCACGTGAGGTCCCAGTATGTGGCCCTGCTGCAGGGAAGTCACGCT
  GACCGCTCCCGAGGCAAGAGGTTCAGCCAGAATTTTCGAGAGGTGGCAGGCAGGTTCCTGATGTCAGAGA
  CCTCCACTCACCTGCTAGTGTTCGGAATGGAGCAGCGGCTGCCGCCTAACAGCGAGCTGGTGCAGGCTGT
  GCTGCGGCTGTTCCAGGAGCCTGTGCCCAGAACAGCTCTCCGGAGGTTTGAGAGGCTGTCCCCACACAGT
  GCCCGGGCTCGGGTCACCATTGAATGGCTGAGAGTCCGTGAGGATGGCTCCAATCGCACTGCCCTCATCG
  ACTCTAGGCTCGTGTCCATCCACGAGAGCGGCTGGAAGGCCTTCGACGTGACCGAGGCCGTGAACTTCTG
  GCAGCAGCTGAGCCGGCCGAGGCAGCCGCTGCTGCTCCAGGTGTCGGTGCAGAGGGAGCATCTGGGGCCG
  GGGACCTGGAGCGCACACAAGTTGGTCCGTTTCGCGGCGCAGGGGACGCCGGACGGCAAGGGGCAGGGCG
  AGCCACAGCTGGAGCTGCACACGCTGGACCTCAAGGACTACGGAGCTCAAGGCAATTGTGACCCCGAGGT
  ACCAGTGACTGAAGGCACCCGATGCTGTCGCCAGGAGATGTACCTGGACCTGCAGGGGATGAAGTGGGCC
  GAGAACTGGATCCTAGAACCGCCAGGGTTCCTGACGTATGAATGTGTGGGCAGCTGCCTGCAGCTACCAG
  AGTCCCTGACCATCGGGTGGCCATTTCTGGGGCCTCGGCAGTGTGTTGCCTCAGAGATGACCTCCTTGCC
  CATGATTGTCAGTGTGAAGGAGGGAGGCAGGACCAGGCCTCAAGTGGTCAGCCTGCCCAACATGAGGGTG
  CAGACCTGTAGCTGCGCCTCAGATGGGGCGCTCATACCCAGGGGGATAGATCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205670 representing NM_177099
  Red=Cloning site Green=Tags(s)
  
  MKSLWLCWALWVLPLAGPGAAMTEEQVLSSLLQQLQLSQAPTLDSADVEEMAIPTHVRSQYVALLQGSHA
  DRSRGKRFSQNFREVAGRFLMSETSTHLLVFGMEQRLPPNSELVQAVLRLFQEPVPRTALRRFERLSPHS
  ARARVTIEWLRVREDGSNRTALIDSRLVSIHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGP
  GTWSAHKLVRFAAQGTPDGKGQGEPQLELHTLDLKDYGAQGNCDPEVPVTEGTRCCRQEMYLDLQGMKWA
  ENWILEPPGFLTYECVGSCLQLPESLTIGWPFLGPRQCVASEMTSLPMIVSVKEGGRTRPQVVSLPNMRV
  QTCSCASDGALIPRGIDL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177099
• ORF Size: 1104 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177099.3, NP_796073.1
• RefSeq Size: 2534
• RefSeq ORF: 1107
• Locus ID: 320202
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This protein is also important in self-renewal and differentiation of mouse embryonic stem cells. Mice lacking a functional copy of this gene exhibit defects in axial and left-right patterning. [provided by RefSeq, Aug 2016]