H2afy (BC006955) Mouse Tagged ORF Clone

CAT#: MG205680

  • TrueORF®

H2afy (GFP-tagged) - Mouse H2A histone family, member Y (cDNA clone MGC:6938 IMAGE:2811919)


Reconstitution Protocol

USD 390.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol H2afy
Synonyms mH2a1, H2AF12M, macroH2A1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG205680 representing BC006955
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGAGCCGCGGCGGGAAGAAGAAATCCACCAAGACCTCCCGGTCAGCCAAGGCCGGAGTCATCTTCC
CTGTGGGACGCATGCTTCGGTACATCAAGAAAGGCCACCCTAAGTATAGGATCGGAGTGGGGGCACCTGT
GTACATGGCTGCTGTCCTGGAGTACCTGACTGCTGAGATCCTGGAGCTGGCTGGCAATGCAGCAAGAGAC
AACAAGAAGGGACGGGTCACACCCCGGCACATCCTGTTAGCTGTGGCCAATGATGAAGAGCTAAACCAGC
TGCTAAAGGGTGTCACCATAGCCAGCGGGGGCGTGTTGCCGAATATCCATCCTGAGTTGCTAGCGAAGAA
GCGAGGATCCAAGGGAAAATTGGAAGCCATCATCACGCCTCCGCCGGCCAAAAAGGCCAAGTCTCCATCC
CAGAAGAAGCCAGTGGCTAAGAAGACAGGAGGCAAGAAAGGGGCCCGGAAGTCTAAGAAGAAGCAGGGAG
AAGTGAGCAAGGCGGCCAGCGCAGACAGTACGACGGAGGGCACGCCTACAGACGGCTTCACTGTCCTCTC
CACCAAGAGCCTCTTCCTCGGCCAGAAGTTGCAAGTTGTTCAGGCTGACATTGCCTCGATCGACAGTGAT
GCTGTCGTTCACCCGACAAACACTGACTTCTACACCGGTGGTGAAGTAGGAAACACACTGGAGAAGAAGG
GCGGCAAGGAGTTTGTAGAAGCTGTTCTGGAACTCCGGAAAAAGAACGGGCCCTTGGAGGTAGCTGGAGC
TGCTATTAGTGCAGGCCATGGCCTGCCTGCCAAGTTTGTGATCCACTGTAATAGTCCTGTCTGGGGTGCA
GACAAATGTGAAGAACTTCTAGAAAAGACGGTGAAAAACTGCTTGGCTCTAGCTGATGACAGAAAGCTGA
AATCCATCGCCTTCCCATCCATTGGCAGCGGCAGGAACGGGTTCCCGAAGCAGACAGCGGCCCAGCTCAT
TCTGAAGGCCATCTCCAGCTACTTTGTCTCCACGATGTCCTCCTCCATCAAAACTGTGTACTTCATGCTT
TTTGACAGTGAGAGCATAGGTATCTATGTGCAGGAAATGGCCAAGCTGGACGCCAAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG205680 representing BC006955
Red=Cloning site Green=Tags(s)

MSSRGGKKKSTKTSRSAKAGVIFPVGRMLRYIKKGHPKYRIGVGAPVYMAAVLEYLTAEILELAGNAARD
NKKGRVTPRHILLAVANDEELNQLLKGVTIASGGVLPNIHPELLAKKRGSKGKLEAIITPPPAKKAKSPS
QKKPVAKKTGGKKGARKSKKKQGEVSKAASADSTTEGTPTDGFTVLSTKSLFLGQKLQVVQADIASIDSD
AVVHPTNTDFYTGGEVGNTLEKKGGKEFVEAVLELRKKNGPLEVAGAAISAGHGLPAKFVIHCNSPVWGA
DKCEELLEKTVKNCLALADDRKLKSIAFPSIGSGRNGFPKQTAAQLILKAISSYFVSTMSSSIKTVYFML
FDSESIGIYVQEMAKLDAN

TRTRRLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC006955
ORF Size 1109 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq BC006955, AAH06955
RefSeq Size 1366
RefSeq ORF 1109
Locus ID 26914
Gene Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2015]

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