Wnt1 (BC005449) Mouse Tagged ORF Clone

CAT#: MG205702

Wnt1 (GFP-tagged) - Mouse wingless-related MMTV integration site 1 (cDNA clone MGC:6192 IMAGE:3493095)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wnt1
• Synonyms: swaying
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205702 representing BC005449
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTCTGGGCGCTGCTGCCCAGCTGGGTTTCTACTACGTTGCTACTGGCACTGACCGCTCTGCCCG
  CAGCCCTAGCTGCCAACAGTAGTGGCCGATGGTGGGGCATCGTGAACATAGCCTCCTCCACGAACCTGTT
  GACGGATTCCAAGAGCCTGCAGCTGGTGCTCGAGCCCAGTCTGCAGCTGCTGAGCCGCAAGCAGCGGCGA
  CTGATCCGACAGAACCCGGGGATCCTGCACAGCGTGAGTGGAGGGCTCCAGAGCGCCGTGCGAGAGTGCA
  AATGGCAATTCCGAAACCGCCGCTGGAACTGCCCCACTGCTCCGGGGCCCCACCTCTTCGGCAAGATCGT
  CAACCGAGGCTGCCGAGAAACAGCGTTCATCTTCGCAATCACCTCCGCCGGGGTCACACATTCCGTGGCG
  CGCTCCTGCTCCGAAGGCTCCATCGAGTCCTGCACCTGCGACTACCGGCGGCGCGGCCCTGGGGGCCCCG
  ACTGGCACTGGGGGGGCTGCAGTGACAACATCGATTTTGGTCGCCTCTTTGGCCGAGAGTTCGTGGACTC
  CGGGGAGAAGGGGCGGGACCTACGCTTCCTCATGAACCTTCACAACAACGAGGCAGGGCGAACGACCGTG
  TTCTCTGAGATGCGCCAAGAGTGCAAATGCCACGGGATGTCCGGCTCCTGCACGGTGCGCACGTGTTGGA
  TGCGGCTGCCCACGCTGCGCGCTGTGGGCGACGTGCTGCGCGACCGCTTCGACGGTGCCTCCCGCGTCCT
  TTACGGCAACAGAGGCAGCAACCGCGCCTCGCGGGCGGAGCTGCTGCGCCTGGAGCCCGAAGACCCCGCG
  CACAAGCCTCCCTCCCCTCACGACCTCGTCTACTTCGAGAAATCGCCCAACTTCTGCACGTACAGTGGCC
  GCCTGGGCACAGCTGGCACAGCTGGACGAGCTTGCAACAGCTCGTCTCCCGCGCTGGACGGCTGTGAGCT
  GCTGTGCTGTGGCCGAGGCCACCGCACGCGCACGCAGCGCGTCACGGAGCGCTGCAACTGCACCTTCCAC
  TGGTGCTGCCACGTCAGCTGCCGCAACTGCACGCACACGCGCGTTCTGCACGAGTGTCTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205702 representing BC005449
  Red=Cloning site Green=Tags(s)
  
  MGLWALLPSWVSTTLLLALTALPAALAANSSGRWWGIVNIASSTNLLTDSKSLQLVLEPSLQLLSRKQRR
  LIRQNPGILHSVSGGLQSAVRECKWQFRNRRWNCPTAPGPHLFGKIVNRGCRETAFIFAITSAGVTHSVA
  RSCSEGSIESCTCDYRRRGPGGPDWHWGGCSDNIDFGRLFGREFVDSGEKGRDLRFLMNLHNNEAGRTTV
  FSEMRQECKCHGMSGSCTVRTCWMRLPTLRAVGDVLRDRFDGASRVLYGNRGSNRASRAELLRLEPEDPA
  HKPPSPHDLVYFEKSPNFCTYSGRLGTAGTAGRACNSSSPALDGCELLCCGRGHRTRTQRVTERCNCTFH
  WCCHVSCRNCTHTRVLHECL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC005449
• ORF Size: 1112 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC005449, AAH05449
• RefSeq Size: 2463
• RefSeq ORF: 1112
• Locus ID: 22408