Popdc2 (BC064005) Mouse Tagged ORF Clone

CAT#: MG205720

Popdc2 (GFP-tagged) - Mouse popeye domain containing 2 (cDNA clone MGC:70008 IMAGE:30287674)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Popdc2
• Synonyms: Pop2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205720 representing BC064005
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCCAATGGCAGCTCGGTGGCTCAGCTCCTCTGGCAGCCGCCCGTGTGCAGGAGCTGGAAACCGG
  ATGTAGAAGGAGCTGTCTATCACCTGGCCAACTGTTTCTTGCTTATGGGCTTTATGGCAGGCAGTGGAGT
  GTATGGATGCTTCTATCTTTTCGGCATCCTGGGCCCAGGCTACCTCTGCTGCGTGCTGTGGGGCTGGTTT
  GATGCTTGTGGACTAGACATCGTCCTTTGGAACGTCCTCCTGACAGTGGCTTGCCTGCTTCAGCTGGCAC
  AGCTGGTCTATCGTGTCCGGGTGAATACCCTCCCGGAGGAATTCAATCTCCTCTACAGGACACTGTGCCT
  GCCCCTGCAGGTGCCCCTGCAGGTCTACAAGGAGATTGTCCACTGCTGCCATGAGCAGGTCTTGACGCTG
  GCCACAGAGCAGACCTACGCTGTGGAGGGGGAGACCCCCATCAATCGCCTGTCCCTGCTCCTCTCAGGCC
  GGGTTCGAGTGAGCCAAGACGGGCAGTTTCTGCACTACATCTTTCCGTATCAGTTCATGGACTCTCCTGA
  ATGGGAATCACTGCATCCTTCTGAAGAGGGGACCTTCCAGGTGACGCTGACTGCGGAGACCGAATGTAGC
  TACATTTCCTGGCCCCGGAAAAATCTTTACCTTCTTCTGAACAGAGAGCGATACATCTCCCGCCTCTTCT
  CAGCCCTGCTGGGCTATGACATCTCGGAGAAGCTCTATACCCTCAATGACAAGCTGTTTGCCAAGTTTGG
  GTTACGCTTCGACATCCGCCTTCCCAGCCTCTACCACGTTCTGAGTCCCTCTGCCTCAGACGGGGAACCA
  GAGTCTGAGAAAGATGATGAAGAAGCCCTTGAGGCAGCTGTGTCCCCTGCTCAGGCCAGGCCCATCTGCA
  TCGTGCCAACACCCCCTTGTTCCGCGCCTCCAGCAACCACCAACTTTCCCGTGCCTCTGCCCCGGGCCAG
  GATGCCCAGGATGCCCAGGCCGGACAGCGGCAACCTGGGTGAGGACTCCACCAGTCTGGTGCTGGAGGAT
  TTTGAGGAGGTTTCAGGATCAGAGTCGTTTATGGATTATAGGAGCGATGGGGAGTACATGAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205720 representing BC064005
  Red=Cloning site Green=Tags(s)
  
  MSANGSSVAQLLWQPPVCRSWKPDVEGAVYHLANCFLLMGFMAGSGVYGCFYLFGILGPGYLCCVLWGWF
  DACGLDIVLWNVLLTVACLLQLAQLVYRVRVNTLPEEFNLLYRTLCLPLQVPLQVYKEIVHCCHEQVLTL
  ATEQTYAVEGETPINRLSLLLSGRVRVSQDGQFLHYIFPYQFMDSPEWESLHPSEEGTFQVTLTAETECS
  YISWPRKNLYLLLNRERYISRLFSALLGYDISEKLYTLNDKLFAKFGLRFDIRLPSLYHVLSPSASDGEP
  ESEKDDEEALEAAVSPAQARPICIVPTPPCSAPPATTNFPVPLPRARMPRMPRPDSGNLGEDSTSLVLED
  FEEVSGSESFMDYRSDGEYMR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC064005
• ORF Size: 1115 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC064005, AAH64005
• RefSeq Size: 2184
• RefSeq ORF: 1115
• Locus ID: 64082
• Gene Summary: This gene encodes a member of the Popeye domain containing family of membrane proteins. Proteins of this family contain three helical transmembrane domains and a conserved intracellular Popeye domain. In the adult mouse, this gene is expressed at high levels in cardiac myocytes, and mice deficient for this gene develop stress-induced cardiac pacemaker dysfunction. The protein binds to a two-pore domain potassium channel and recruits it to the plasma membrane. Cyclic adenosine monophosphate negatively regulates this interaction through the Popeye domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]