Ggta1 (BC006810) Mouse Tagged ORF Clone

CAT#: MG205759

Ggta1 (GFP-tagged) - Mouse glycoprotein galactosyltransferase alpha 1, 3 (cDNA clone MGC:11545 IMAGE:3154188)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ggta1
• Synonyms: GALT, Gal, alpha3GalT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205759 representing BC006810
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCACTATGCTTCAAGATCTCCATGTCAACAAGATCTCCATGTCAAGATCCAAGTCAGAAACAAGTC
  TTCCATCCTCAAGATCTGGATCACAGGAGAAAATAATGAATGTCAAGGGAAAAGTAATCCTGTTGATGCT
  GATTGTCTCAACCGTGGTTGTCGTGTTTTGGGAATATGTCAACAGGACCCACAGTTATCAAGAAGACAAC
  GTAGAAGGACGGAGAGAAAAGGGTAGAAATGGAGATCGCATTGAAGAGCCTCAGCTATGGGACTGGTTCA
  ATCCAAAGAACCGCCCGGATGTTTTGACAGTGACCCCGTGGAAGGCGCCGATTGTGTGGGAAGGCACTTA
  TGACACAGCTCTGCTGGAAAAGTACTACGCCACACAGAAACTCACTGTGGGGCTGACAGTGTTTGCTGTG
  GGAAAGTACATTGAGCATTACTTAGAAGACTTTCTGGAGTCTGCTGACATGTACTTCATGGTTGGCCATC
  GGGTCATATTTTACGTCATGATAGACGACACCTCCCGGATGCCTGTCGTGCACCTGAACCCTCTACATTC
  CTTACAAGTCTTTGAGATCAGGTCTGAGAAGAGGTGGCAGGATATCAGCATGATGCGCATGAAGACCATT
  GGGGAGCACATCCTGGCCCACATCCAGCACGAGGTCGACTTCCTCTTCTGCATGGACGTGGATCAAGTCT
  TTCAAGACAACTTCGGGGTGGAAACTCTGGGCCAGCTGGTAGCACAGCTCCAGGCCTGGTGGTACAAGGC
  CAGTCCCGAGAAGTTCACCTATGAGAGGCGGGAACTGTCGGCCGCGTACATTCCATTCGGAGAGGGGGAT
  TTTTACTACCACGCGGCCATTTTTGGAGGAACGCCTACTCACATTCTCAACCTCACCAGGGAGTGCTTTA
  AGGGGATCCTCCAGGACAAGAAACATGACATAGAAGCCCAGTGGCATGATGAGAGCCACCTCAACAAATA
  CTTCCTTTTCAACAAACCCACTAAAATCCTATCTCCAGAGTATTGCTGGGACTATCAGATAGGCCTGCCT
  TCAGATATTAAAAGTGTCAAGGTAGCTTGGCAGACAAAAGAGTATAATTTGGTTAGAAATAATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205759 representing BC006810
  Red=Cloning site Green=Tags(s)
  
  MITMLQDLHVNKISMSRSKSETSLPSSRSGSQEKIMNVKGKVILLMLIVSTVVVVFWEYVNRTHSYQEDN
  VEGRREKGRNGDRIEEPQLWDWFNPKNRPDVLTVTPWKAPIVWEGTYDTALLEKYYATQKLTVGLTVFAV
  GKYIEHYLEDFLESADMYFMVGHRVIFYVMIDDTSRMPVVHLNPLHSLQVFEIRSEKRWQDISMMRMKTI
  GEHILAHIQHEVDFLFCMDVDQVFQDNFGVETLGQLVAQLQAWWYKASPEKFTYERRELSAAYIPFGEGD
  FYYHAAIFGGTPTHILNLTRECFKGILQDKKHDIEAQWHDESHLNKYFLFNKPTKILSPEYCWDYQIGLP
  SDIKSVKVAWQTKEYNLVRNNV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC006810
• ORF Size: 1118 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC006810, AAH06810
• RefSeq Size: 3315
• RefSeq ORF: 1118
• Locus ID: 14594
• Gene Summary: This gene encodes a member of the galactosyltransferase family of intracellular, membrane-bound enzymes that are involved in the biosynthesis of glycoproteins and glycolipids. The encoded protein catalyzes the transfer of galactose from UDP-galactose to N-acetyllactosamine in an alpha(1,3) linkage to form galactose alpha(1,3)-galactose. Mice lacking the encoded protein develop cortical cataracts. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2015]