Hsd3b1 (NM_008293) Mouse Tagged ORF Clone

CAT#: MG205766

Hsd3b1 (GFP-tagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hsd3b1
• Synonyms: 3-beta-HSD I; D3Ertd383e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205766 representing NM_008293
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGATGGAGCTGCCTGGTGACAGGAGCAGGAGGGTTTGTGGGCCAGAGGATCATCAAGATGTTGG
  TGCAGGAGAAAGAACTGCAGGAGGTCAGAGCTCTGGACAAAGTATTCCGACCAGAAACCAAGGAGGAATT
  CTCCAAGCTGCAGACAAAGACCAAGGTGACAGTGTTGGAAGGAGACATTCTGGATGCCCAGTGCCTGAGG
  AGAGCCTGCCAGGGCATCTCTGTTGTCATCCACACTGCTGCTGTCATTGATGTCACAGGTGTCATTCCCA
  GGCAGACCATCCTAGATGTCAATCTGAAAGGTACCCAGAACCTATTGGAGGCCTGTGTTCAAGCAAGTGT
  GCCAGCCTTCATCTTCTGCAGCTCAGTTGATGCTGCAGGGCCCAACTCGTACAAGAAGATCGTCCTGAAT
  GGCCACGAGGAACAGAATCATGAAAGCACATGGTCTGATCCATACCCATACAGCAAAAAGATGGCTGAGA
  AGGCAGTACTGGCAGCCAATGGGAGCATGCTGAAAAATGGTGGCACTTTGAATACTTGTGCCTTAAGACC
  CATGTACATTTATGGGGAGAGAAGTCCATTCATTTTTAATGCAATAATTAGGGCCCTCAAAAATAAGGGT
  ATTCTGTGTGTTACTGGCAAATTCTCCATAGCCAACCCAGTATATGTGGAAAATGTGGCCTGGGCACACA
  TTCTGGCAGCCAGGGGCCTTCGAGACCCCAAGAAGTCTACAAGCATCCAAGGACAGTTCTACTACATCTC
  AGATGACACCCCTCACCAAAGCTATGATGATTTAAATTACACCCTGAGTAAGGAATGGGGGCTCCGCCCT
  AATGCCAGCTGGAGCCTTCCTCTGCCCCTGCTCTACTGGCTTGCCTTCCTGCTGGAAACTGTGAGCTTCC
  TGCTACGTCCAGTCTACAGGTATAGACCTCTCTTTAACCGCCACTCGATCACACTGTCAAATAGCACGTT
  CACTTTTTCTTACAAGAAAGCTCAGCGAGATCTGGGCTATGAGCCACTTGTCAACTGGGAGGAAGCAAAG
  CAGAAAACCTCAGAGTGGATAGGGACAATAGTGGAACAGCACAGGGAGATATTGGACACAAAGTGCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205766 representing NM_008293
  Red=Cloning site Green=Tags(s)
  
  MAGWSCLVTGAGGFVGQRIIKMLVQEKELQEVRALDKVFRPETKEEFSKLQTKTKVTVLEGDILDAQCLR
  RACQGISVVIHTAAVIDVTGVIPRQTILDVNLKGTQNLLEACVQASVPAFIFCSSVDAAGPNSYKKIVLN
  GHEEQNHESTWSDPYPYSKKMAEKAVLAANGSMLKNGGTLNTCALRPMYIYGERSPFIFNAIIRALKNKG
  ILCVTGKFSIANPVYVENVAWAHILAARGLRDPKKSTSIQGQFYYISDDTPHQSYDDLNYTLSKEWGLRP
  NASWSLPLPLLYWLAFLLETVSFLLRPVYRYRPLFNRHSITLSNSTFTFSYKKAQRDLGYEPLVNWEEAK
  QKTSEWIGTIVEQHREILDTKCQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008293
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008293.2, NP_032319.1
• RefSeq Size: 1853
• RefSeq ORF: 1122
• Locus ID: 15492
• Gene Summary: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. [UniProtKB/Swiss-Prot Function]