Casp4 (NM_007609) Mouse Tagged ORF Clone

CAT#: MG205770

Casp4 (GFP-tagged) - Mouse caspase 4, apoptosis-related cysteine peptidase (Casp4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Casp4
• Synonyms: CASP-4; CASP-11; Casp11; Caspl; ich-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205770 representing NM_007609
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAAACAAACACCCTGACAAACCACTTAAGGTGTTGGAACAGCTGGGCAAAGAAGTCCTTACGG
  AGTACCTAGAAAAATTAGTACAAAGCAATGTACTGAAATTAAAGGAGGAAGATAAACAAAAATTTAACAA
  TGCTGAACGCAGTGACAAGCGTTGGGTTTTTGTAGATGCCATGAAAAAGAAACACAGCAAAGTAGGTGAA
  ATGCTTCTCCAGACATTCTTCAGTGTGGACCCAGGCAGCCACCATGGTGAAGCTAATCTGGAAATGGAGG
  AACCAGAAGAATCATTGAACACTCTCAAGCTTTGTTCCCCTGAAGAGTTCACAAGGCTTTGCAGAGAAAA
  GACACAAGAAATTTACCCAATAAAGAAGGCCAATGGCCGTACACGAAAGGCTCTTATCATATGCAATACA
  GAGTTCAAACATCTCTCACTGAGGTATGGGGCTAACTTTGACATCATTGGTATGAAAGGCCTTCTTGAAG
  ACTTAGGCTACGATGTGGTGGTGAAAGAGGAGCTTACAGCAGAGGGCATGGAGTCAGAGATGAAAGACTT
  TGCTGCACTCTCAGAACACCAGACATCAGACAGCACATTCCTGGTGCTAATGTCTCATGGCACACTGCAT
  GGCATTTGTGGAACAATGCACAGTGAAAAAACTCCAGATGTGCTACAGTATGATACCATCTATCAGATAT
  TCAACAATTGCCACTGTCCAGGTCTACGAGACAAACCCAAAGTCATCATTGTGCAGGCCTGCAGAGGTGG
  GAACTCTGGAGAAATGTGGATCAGAGAGTCTTCAAAACCCCAGTTGTGCAGAGGTGTAGATCTACCTAGG
  AATATGGAAGCTGATGCTGTCAAGCTGAGCCACGTGGAGAAGGACTTCATTGCCTTCTACTCTACAACCC
  CACATCACTTGTCCTACCGAGACAAAACAGGAGGCTCTTACTTCATCACTAGACTCATTTCCTGCTTCCG
  GAAACATGCTTGCTCTTGTCATCTCTTTGATATATTCCTGAAGGTGCAACAATCATTTGAAAAGGCAAGT
  ATTCATTCCCAGATGCCCACCATTGATCGGGCAACCTTGACGAGATATTTCTACCTCTTTCCTGGCAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205770 representing NM_007609
  Red=Cloning site Green=Tags(s)
  
  MAENKHPDKPLKVLEQLGKEVLTEYLEKLVQSNVLKLKEEDKQKFNNAERSDKRWVFVDAMKKKHSKVGE
  MLLQTFFSVDPGSHHGEANLEMEEPEESLNTLKLCSPEEFTRLCREKTQEIYPIKKANGRTRKALIICNT
  EFKHLSLRYGANFDIIGMKGLLEDLGYDVVVKEELTAEGMESEMKDFAALSEHQTSDSTFLVLMSHGTLH
  GICGTMHSEKTPDVLQYDTIYQIFNNCHCPGLRDKPKVIIVQACRGGNSGEMWIRESSKPQLCRGVDLPR
  NMEADAVKLSHVEKDFIAFYSTTPHHLSYRDKTGGSYFITRLISCFRKHACSCHLFDIFLKVQQSFEKAS
  IHSQMPTIDRATLTRYFYLFPGN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007609
• ORF Size: 1119 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007609.1, NP_031635.1
• RefSeq Size: 1433
• RefSeq ORF: 1122
• Locus ID: 12363
• Gene Summary: This gene encodes a member of the cysteine proteases that plays important roles in apoptosis, cell migration and the inflammatory response. The encoded protein mediates production of pro-inflammatory cytokines by macrophages upon bacterial infection. Mice lacking the encoded protein are resistant to endotoxic shock induced by lipopolysaccharide. A 5-bp deletion encompassing a splice acceptor junction resulting in alternate splicing and a shorter non-functional isoform in certain mouse strains has been described. Although its official nomenclature is "caspase 4, apoptosis-related cysteine peptidase", this gene and its encoded protein have historically been called caspase 11. This gene is present in a cluster of three caspase genes on chromosome 9. [provided by RefSeq, Apr 2015]