Casp4 (NM_007609) Mouse Tagged ORF Clone

CAT#: MR205770

Casp4 (Myc-DDK-tagged) - Mouse caspase 4, apoptosis-related cysteine peptidase (Casp4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Casp4
• Synonyms: CASP-4; CASP-11; Casp11; Caspl; ich-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205770 representing NM_007609
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAAACAAACACCCTGACAAACCACTTAAGGTGTTGGAACAGCTGGGCAAAGAAGTCCTTACGG
  AGTACCTAGAAAAATTAGTACAAAGCAATGTACTGAAATTAAAGGAGGAAGATAAACAAAAATTTAACAA
  TGCTGAACGCAGTGACAAGCGTTGGGTTTTTGTAGATGCCATGAAAAAGAAACACAGCAAAGTAGGTGAA
  ATGCTTCTCCAGACATTCTTCAGTGTGGACCCAGGCAGCCACCATGGTGAAGCTAATCTGGAAATGGAGG
  AACCAGAAGAATCATTGAACACTCTCAAGCTTTGTTCCCCTGAAGAGTTCACAAGGCTTTGCAGAGAAAA
  GACACAAGAAATTTACCCAATAAAGAAGGCCAATGGCCGTACACGAAAGGCTCTTATCATATGCAATACA
  GAGTTCAAACATCTCTCACTGAGGTATGGGGCTAACTTTGACATCATTGGTATGAAAGGCCTTCTTGAAG
  ACTTAGGCTACGATGTGGTGGTGAAAGAGGAGCTTACAGCAGAGGGCATGGAGTCAGAGATGAAAGACTT
  TGCTGCACTCTCAGAACACCAGACATCAGACAGCACATTCCTGGTGCTAATGTCTCATGGCACACTGCAT
  GGCATTTGTGGAACAATGCACAGTGAAAAAACTCCAGATGTGCTACAGTATGATACCATCTATCAGATAT
  TCAACAATTGCCACTGTCCAGGTCTACGAGACAAACCCAAAGTCATCATTGTGCAGGCCTGCAGAGGTGG
  GAACTCTGGAGAAATGTGGATCAGAGAGTCTTCAAAACCCCAGTTGTGCAGAGGTGTAGATCTACCTAGG
  AATATGGAAGCTGATGCTGTCAAGCTGAGCCACGTGGAGAAGGACTTCATTGCCTTCTACTCTACAACCC
  CACATCACTTGTCCTACCGAGACAAAACAGGAGGCTCTTACTTCATCACTAGACTCATTTCCTGCTTCCG
  GAAACATGCTTGCTCTTGTCATCTCTTTGATATATTCCTGAAGGTGCAACAATCATTTGAAAAGGCAAGT
  ATTCATTCCCAGATGCCCACCATTGATCGGGCAACCTTGACGAGATATTTCTACCTCTTTCCTGGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205770 representing NM_007609
  Red=Cloning site Green=Tags(s)
  
  MAENKHPDKPLKVLEQLGKEVLTEYLEKLVQSNVLKLKEEDKQKFNNAERSDKRWVFVDAMKKKHSKVGE
  MLLQTFFSVDPGSHHGEANLEMEEPEESLNTLKLCSPEEFTRLCREKTQEIYPIKKANGRTRKALIICNT
  EFKHLSLRYGANFDIIGMKGLLEDLGYDVVVKEELTAEGMESEMKDFAALSEHQTSDSTFLVLMSHGTLH
  GICGTMHSEKTPDVLQYDTIYQIFNNCHCPGLRDKPKVIIVQACRGGNSGEMWIRESSKPQLCRGVDLPR
  NMEADAVKLSHVEKDFIAFYSTTPHHLSYRDKTGGSYFITRLISCFRKHACSCHLFDIFLKVQQSFEKAS
  IHSQMPTIDRATLTRYFYLFPGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007609
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007609.1, NM_007609.2, NM_007609.3, NP_031635.1
• RefSeq Size: 1350
• RefSeq ORF: 1122
• Locus ID: 12363
• MW: 43.2 kDa
• Gene Summary: This gene encodes a member of the cysteine proteases that plays important roles in apoptosis, cell migration and the inflammatory response. The encoded protein mediates production of pro-inflammatory cytokines by macrophages upon bacterial infection. Mice lacking the encoded protein are resistant to endotoxic shock induced by lipopolysaccharide. A 5-bp deletion encompassing a splice acceptor junction resulting in alternate splicing and a shorter non-functional isoform in certain mouse strains has been described. Although its official nomenclature is "caspase 4, apoptosis-related cysteine peptidase", this gene and its encoded protein have historically been called caspase 11. This gene is present in a cluster of three caspase genes on chromosome 9. [provided by RefSeq, Apr 2015]