Actg2 (BC002042) Mouse Tagged ORF Clone

CAT#: MG205841

Actg2 (GFP-tagged) - Mouse actin, gamma 2, smooth muscle, enteric (cDNA clone MGC:6022 IMAGE:3591323)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Actg2
• Synonyms: SMGA, ACTA3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205841 representing BC002042
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGAAGAAGAGACCACCGCCCTTGTGTGTGACAATGGCTCTGGCCTGTGCAAGGCAGGCTTTGCAG
  GAGATGATGCCCCCAGGGCTGTCTTCCCCTCCATTGTGGGCCGCCCTAGACATCAGGGTGTGATGGTGGG
  AATGGGCCAGAAAGACAGCTATGTGGGGGACGAGGCTCAGAGCAAGCGTGGGATCCTAACTCTCAAGTAC
  CCTATTGAACATGGCATCATCACCAACTGGGATGACATGGAGAAGATCTGGCACCACTCCTTCTACAATG
  AGCTTCGAGTAGCACCAGAAGAGCACCCCACCCTGCTCACAGAGGCCCCCCTAAACCCCAAAGCAAACAG
  GGAGAAGATGACCCAGATCATGTTCGAAACCTTCAATGTCCCTGCCATGTATGTTGCTATTCAGGCTGTG
  CTCTCACTCTATGCATCCGGCCGTACCACAGGCATCGTCCTGGATTCGGGGGATGGCGTCACCCACAATG
  TCCCCATCTATGAGGGCTATGCACTGCCCCATGCCATCATGCGTCTTGACCTGGCTGGACGGGATCTCAC
  AGACTACCTCATGAAGATTCTCACAGAAAGAGGCTATTCCTTTGTGACCACAGCTGAGAGAGAAATTGTA
  CGAGACATCAAGGAGAAGCTGTGCTATGTAGCCCTGGATTTCGAGAATGAGATGGCCACAGCAGCTTCAT
  CTTCTTCCCTGGAGAAAAGCTACGAGTTGCCTGATGGGCAGGTCATCACTATTGGCAACGAACGCTTCCG
  CTGCCCGGAGACCCTCTTCCAGCCTTCCTTCATTGGCATGGAGTCAGCTGGAATTCATGAAACAACATAC
  AATTCCATCATGAAGTGTGACATTGACATCCGCAAAGATTTGTATGCTAACAATGTCCTCTCTGGGGGCA
  CTACCATGTACCCTGGCATTGCTGACAGGATGCAGAAGGAAATCACAGCCTTGGCTCCCAGCACCATGAA
  GATCAAGATTATCGCTCCTCCTGAGCGGAAGTACTCAGTCTGGATTGGCGGCTCCATCCTGGCCTCTCTC
  TCCACCTTCCAGCAAATGTGGATCAGCAAGCCAGAGTATGATGAGGCAGGGCCCTCCATTGTCCACAGGA
  AATGCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205841 representing BC002042
  Red=Cloning site Green=Tags(s)
  
  MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKY
  PIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAV
  LSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIV
  RDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTY
  NSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASL
  STFQQMWISKPEYDEAGPSIVHRKCF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC002042
• ORF Size: 1130 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC002042, AAH02042
• RefSeq Size: 1319
• RefSeq ORF: 1130
• Locus ID: 11468
• Gene Summary: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProtKB/Swiss-Prot Function]