Oas1b (BC012877) Mouse Tagged ORF Clone

CAT#: MG205844

Oas1b (GFP-tagged) - Mouse 2'-5' oligoadenylate synthetase 1B (cDNA clone MGC:19005 IMAGE:4016177)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Oas1b
• Synonyms: Mmu-L1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205844 representing BC012877
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGGATCTGAGGAGCATCCCGGCCTCGAAGCTTGATAAGTTCATAGAGAATCATCTCCCGGACA
  CCAGCTTCTGTGCTGACCTCAGAGAAGTCATAGATGCCCTGTGTGCTCTCCTGAAGGACAGATTCTTCCG
  GGGCCCCGTCCGCCGAATGAGGGCCTCTAAGGGGGTCAAGGGCAAATGCACCGCGCTCAAGGGCAGGTCA
  GACGCTGACCTGGTGGTGTTCCTTAACAATCTCACCTACTTTGAGGATCAATTAAACCAACAGGGAGTGT
  TGATTAAGGAAATTAAGAAACAGCTGTACGAGGTTCAGCATGAGAGACGTTTTGGAGTCAAGTTTGAGGT
  CCAGAGTTTAAGGAGTCCCAACTCCCGGGCTCTGAGCTTCAAGCTGAGCGCCCCCGACCTGCTGAAGGAG
  GTGAAGTTTGACGTGCTGCCAGCCTATGATTTACTGGATCATCTTAACATCCTCAAGAAGCCTAACCAAC
  AATTCTACGCCAATCTCATCAGTGGGCGTACCCCGCTGGGGAAGGAGGGCAAGTTATTGACCTGCTTTAT
  GGGGCTTCGGAAGTACTTCCTGAACTGTCGCCCAACCAAGCTGAAGCGCCTCATCCACCTGGTCACGCAC
  TGGTACCAACTGTGTAAGGAGAAGCTGGGGGACCCGCTGCCCCCACAGTATGCCCTGGAGCTGCTCACAG
  TCTATGCCTGGGAGTATGGGAGTCGAGTAACTAAATTCAACACAGCCCAGGGCTTCCGAACCGTCTTGGA
  ACTGGTTACCAAGTACAAACAGCTTCGAATCTACTGGACAGTGTATTATGACTTTCGACATCAAGAGGTC
  TCTGAATACCTGCACCAACAGCTCAAAAAAGACAGGCCTGTGATCTTGGACCCCGCTGATCCAACAAGGA
  ATATAGCTGGTTTGAACCCAAAGGACTGGCGGCGTCTAGCAGGAGAGGCTGCCGCCTGGCTGCAATACCC
  ATGCTTTAAGTACAGGGACGGTTCCCCAGTGTGCTCCTGGGAGGTGCCGACGGAGGTTGCAGTGCCAACG
  AAGTATCTCTTTTGTCGTATTTTCTGGTTATTGTTTTGGTCTTTGTTTCATTTCATCTTTGGGAAGACTT
  CATCTGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205844 representing BC012877
  Red=Cloning site Green=Tags(s)
  
  MEQDLRSIPASKLDKFIENHLPDTSFCADLREVIDALCALLKDRFFRGPVRRMRASKGVKGKCTALKGRS
  DADLVVFLNNLTYFEDQLNQQGVLIKEIKKQLYEVQHERRFGVKFEVQSLRSPNSRALSFKLSAPDLLKE
  VKFDVLPAYDLLDHLNILKKPNQQFYANLISGRTPLGKEGKLLTCFMGLRKYFLNCRPTKLKRLIHLVTH
  WYQLCKEKLGDPLPPQYALELLTVYAWEYGSRVTKFNTAQGFRTVLELVTKYKQLRIYWTVYYDFRHQEV
  SEYLHQQLKKDRPVILDPADPTRNIAGLNPKDWRRLAGEAAAWLQYPCFKYRDGSPVCSWEVPTEVAVPT
  KYLFCRIFWLLFWSLFHFIFGKTSSG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC012877
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC012877.1
• RefSeq Size: 1944
• RefSeq ORF: 1130
• Locus ID: 23961
• Gene Summary: This gene is a member of the 2'-5' oligoA synthetase family, which are clustered on chromosome 5. The encoded protein functions in the interferon-regulated OAS/RNase L system, which mediates RNA decay as part of the innate antiviral immunity pathway. The protein binds double-stranded RNA and oligomerizes ATP, which activate the single-stranded RNA cleavage enzyme RNase L. This protein mediates resistance to flaviviruses such as West Nile virus. The majority of wild mouse strains produce a functional protein and are resistant to flavivirus infection, whereas some inbred mouse strains including the strain of the reference genome, C57BL/6J, contain a premature stop codon that inactivates this gene product. [provided by RefSeq, Jul 2008]