Galt (BC010985) Mouse Tagged ORF Clone

CAT#: MG205914

Galt (GFP-tagged) - Mouse galactose-1-phosphate uridyl transferase (cDNA clone MGC:14011 IMAGE:4038194)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Galt
• Synonyms: AW553376
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205914 representing BC010985
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCATAGCGGAGCGGATCCTGAGCAGCGCCAGCAGGCTTCAGAGGCGGACGCCATGGCAGCGACCT
  TCCGGGCGAGCGAACACCAGCATATTCGCTACAACCCGCTCCAGGACGAGTGGGTGTTAGTGTCGGCTCA
  TCGCATGAAGCGGCCCTGGCAAGGACAAGTGGAGCCCCAGCTTCTAAAGACAGTGCCCCGCCACGACCCA
  CTCAACCCTCTGTGTCCCGGGGCCACACGAGCTAATGGGGAGGTGAATCCCCACTATGATGGCACCTTTC
  TGTTTGACAATGACTTCCCGGCTCTGCAGCCCGATGCTCCGGATCCAGGAACCAGTGACCACCCTCTCTT
  CCGAGCAGAGGCCGCCAGAGGAGTTTGTAAGGTCATGTGCTTCCACCCCTGGTCGGATGTGACGCTGCCA
  CTCATGTCTGTCCCTGAGATCCGAGCTGTCATCGATGCATGGGCCTCAGTAACAGAGGAGCTGGGTGCCC
  AGTACCCTTGGGTGCAGATCTTTGAAAATAAAGGAGCCATGATGGGCTGTTCTAACCCCCATCCCCACTG
  CCAGGTTTGGGCTAGCAGCTTCCTGCCAGATATCGCCCAGCGTGAAGAGCGATCCCAGCAGACCTATCAC
  AGCCAGCATGGAAAACCTTTGTTATTGGAATATGGTCACCAAGAGCTCCTCAGGAAGGAACGTCTGGTCC
  TAACCAGTGAGCACTGGATAGTTCTGGTCCCCTTCTGGGCAGTGTGGCCTTTCCAGACACTTCTGCTGCC
  CCGGCGGCACGTGCGGCGGCTACCTGAGCTGAACCCCGCTGAGCGTGATGATCTCGCCTCCATCATGAAG
  AAGCTCTTGACCAAGTACGACAATCTATTTGAGACATCCTTTCCCTACTCCATGGGCTGGCATGGGGCTC
  CCACGGGATTAAAGACTGGAGCCACCTGTGACCACTGGCAGCTCCACGCCCACTACTACCCCCCACTTCT
  GCGATCCGCAACTGTCCGGAAGTTCATGGTTGGCTATGAAATGCTTGCCCAGGCCCAGCGTGACCTCACT
  CCCGAACAGGCCGCAGAAAGATTAAGGGCACTTCCCGAGGTACACTATTGCCTGGCGCAGAAAGACAAGG
  AAACGGCAGCCATTGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205914 representing BC010985
  Red=Cloning site Green=Tags(s)
  
  MSHSGADPEQRQQASEADAMAATFRASEHQHIRYNPLQDEWVLVSAHRMKRPWQGQVEPQLLKTVPRHDP
  LNPLCPGATRANGEVNPHYDGTFLFDNDFPALQPDAPDPGTSDHPLFRAEAARGVCKVMCFHPWSDVTLP
  LMSVPEIRAVIDAWASVTEELGAQYPWVQIFENKGAMMGCSNPHPHCQVWASSFLPDIAQREERSQQTYH
  SQHGKPLLLEYGHQELLRKERLVLTSEHWIVLVPFWAVWPFQTLLLPRRHVRRLPELNPAERDDLASIMK
  KLLTKYDNLFETSFPYSMGWHGAPTGLKTGATCDHWQLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLT
  PEQAAERLRALPEVHYCLAQKDKETAAIA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC010985
• ORF Size: 1139 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC010985, AAH10985
• RefSeq Size: 1300
• RefSeq ORF: 1139
• Locus ID: 14430
• Gene Summary: The protein encoded by this gene is the second enzyme in the Leloir pathway, the metabolic pathway for D-galactose catabolism. It catalyzes the conversion of galactose-1-phosphate and uridine diphosphate-glucose to glucose-1-phosphate and uridine diphosphate galactose. Deficiency of this enzyme causes the genetic metabolic disorder galactosemia. Mice lacking this protein accumulate high levels of galactose and galactose-1 phosphate but are viable and fertile. This protein is negatively regulated through signaling by the polypeptide hormone prolactin, specifically via the short isoform of the prolactin receptor and the transcription factor Forkhead box O3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]