Rnf13 (BC058182) Mouse Tagged ORF Clone

CAT#: MG205943

Rnf13 (GFP-tagged) - Mouse ring finger protein 13 (cDNA clone MGC:68150 IMAGE:5324040)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rnf13
• Synonyms: Rzf
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205943 representing BC058182
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTCTCCATTGGGATGCTCATGCTGTCCGCCACACAGGTCTACACCATCTTGACTGTGCAGCTCT
  TTGCATTCCTAAACCTGCTGCCTGTAGAAGCAGACATTTTAGCATATAATTTTGAAAATGCATCTCAGAC
  ATTTGAGGACCTTCCAGCAAGATTTGGTTATAGACTACCAGCTGAAGGCTTAAAGGGTTTTCTGATTAAC
  TCAAAACCAGAGAATGCGTGTGAACCCATAGTGCCTCCACCACTGAAAGACAATTCTTCCGGCACGTTCA
  TCGTGCTGATTAGAAGACTTGATTGCAATTTTGATATAAAGGTTTTAAATGCACAGAGAGCAGGATACAA
  AGCAGCCATAGTTCACAATGTAGACTCTGATGACCTGATTAGCATGGGATCCAACGACATTGATACACTA
  AAGAAAATTGACATTCCGTCTGTCTTTATTGGTGAATCATCAGCTAATTCCCTGAAAGATGAATTCACAT
  ATGAAAAAGGGGGCCACATTATCTTAGTTCCAGAACTGAGTCTTCCTTTGGAGTACTATCTGATCCCCTT
  CCTCATCATAGTGGGCATCTGCCTCATCTTAATAGTCATTTTCATGATCACAAAATTTGTTCAGGACAGA
  CATAGAAACAGAAGAAATAGACTTCGTAAAGATCAACTTAAAAAACTTCCCGTACATAAATTTAAAAAGG
  GAGATGAGTACGATGTGTGTGCTATCTGTCTGGAGGAGTATGAAGACGGAGATAAGCTAAGGATCCTCCC
  CTGCTCCCATGCTTATCATTGCAAGTGTGTAGATCCCTGGCTAACTAAAACCAAAAAGACCTGTCCAGTC
  TGCAAACAAAAGGTTGTTCCTTCCCAAGGTGACTCGGACTCTGACACAGACAGCAGTCAGGAAGAAAACC
  AAGTGTCAGAGCACACCCCTTTACTTCCACCCTCAGCTTCTGCCAGGACCCAGTCATTTGGGTCTCTGTC
  AGAATCCCACTCACATCACAACATGACAGAGTCTTCAGACTACGAGGACGACGACAATGAAGAGACCGAC
  AGCAGTGACGCAGACAACGAAATTACCGACCACAGTGTTGTGGTCCAGCTGCAGCCGAACGGTGAGCAGG
  ATTACAACATAGCAAATACTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205943 representing BC058182
  Red=Cloning site Green=Tags(s)
  
  MLLSIGMLMLSATQVYTILTVQLFAFLNLLPVEADILAYNFENASQTFEDLPARFGYRLPAEGLKGFLIN
  SKPENACEPIVPPPLKDNSSGTFIVLIRRLDCNFDIKVLNAQRAGYKAAIVHNVDSDDLISMGSNDIDTL
  KKIDIPSVFIGESSANSLKDEFTYEKGGHIILVPELSLPLEYYLIPFLIIVGICLILIVIFMITKFVQDR
  HRNRRNRLRKDQLKKLPVHKFKKGDEYDVCAICLEEYEDGDKLRILPCSHAYHCKCVDPWLTKTKKTCPV
  CKQKVVPSQGDSDSDTDSSQEENQVSEHTPLLPPSASARTQSFGSLSESHSHHNMTESSDYEDDDNEETD
  SSDADNEITDHSVVVQLQPNGEQDYNIANTV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC058182
• ORF Size: 1145 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC058182, AAH58182
• RefSeq Size: 2240
• RefSeq ORF: 1145
• Locus ID: 24017
• Gene Summary: This gene encodes a member of the PA-TM-RING family of proteins that contain a protease associated (PA) domain and a RING finger domain separated by a transmembrane (TM) domain. The encoded protein is an E3 ubiquitin ligase localized to the endosomal-lysosomal vesicles and inner nuclear membrane. Mice lacking the encoded protein have impaired learning abilities associated with a decreased synaptic vesicle density and dysregulated SNARE complex assembly. Alternative splicing of this gene results in multiple transcript variants. A pseudogene for this gene has been identified on the X chromosome. [provided by RefSeq, Jan 2015]