Ckb (NM_021273) Mouse Tagged ORF Clone

CAT#: MG205953

Ckb (GFP-tagged) - Mouse creatine kinase, brain (Ckb)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ckb
• Synonyms: B-CK; Bck; Ck-3; Ck3; Ckbb; CPK-B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205953 representing NM_021273
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTTCTCCAACAGCCATAATACGCAGAAGCTGCGCTTCCCGGCCGAGGATGAGTTCCCTGATCTGA
  GCAGCCACAACAACCATATGGCCAAGGTGCTGACCCCGGAGCTGTACGCCGAGCTCCGTGCCAAGTGCAC
  GCCGAGCGGCTTTACTTTGGACGACGCCATTCAGACTGGCGTAGACAATCCGGGCCACCCGTACATCATG
  ACTGTGGGTGCAGTGGCGGGCGACGAGGAGAGTTACGACGTATTCAAGGACCTCTTCGACCCCATTATTG
  AGGAGCGGCACGGCGGCTACCAGCCCAGTGATGAGCACAAGACCGACCTCAACCCAGACAACCTGCAGGG
  TGGCGATGACCTGGACCCCAACTACGTGCTGAGCTCGCGAGTGCGCACAGGCCGCAGCATCCGCGGCTTC
  TGTCTCCCCCCGCACTGCAGCCGCGGGGAGCGCCGCGCCATCGAGAAGCTGGCAGTAGAAGCTCTGTCCA
  GCCTAGATGGCGACCTGTCTGGCAGGTACTACGCGCTCAAGAGCATGACTGAGGCGGAGCAGCAGCAGCT
  CATTGACGACCACTTCCTCTTCGATAAGCCTGTGTCGCCTCTGCTGCTGGCCTCCGGCATGGCCCGCGAC
  TGGCCGGATGCTCGTGGCATATGGCACAATGACAATAAGACTTTCCTGGTGTGGATTAACGAGGAGGACC
  ACCTGCGAGTCATCTCCATGCAGAAGGGGGGCAACATGAAGGAAGTGTTCACCCGATTCTGCACCGGCCT
  CACTCAGATCGAAACTCTCTTCAAGTCCAAGAACTATGAGTTCATGTGGAATCCTCACCTGGGCTACATC
  CTCACATGCCCATCCAACCTGGGCACCGGACTGCGGGCAGGTGTGCACATCAAGCTGCCCCACCTGGGGA
  AGCACGAGAAGTTCTCGGAGGTGCTCAAGCGGCTGCGGCTTCAGAAGCGAGGCACAGGTGGCGTGGACAC
  CGCTGCTGTCGGTGGGGTGTTTGATGTCTCCAACGCTGACCGCCTGGGCTTCTCGGAGGTGGAACTGGTG
  CAGATGGTGGTGGACGGAGTGAAACTACTCATTGAGATGGAGCAACGGCTCGAGCAGGGTCAGGCAATCG
  ATGACCTCATGCCGGCCCAGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205953 representing NM_021273
  Red=Cloning site Green=Tags(s)
  
  MPFSNSHNTQKLRFPAEDEFPDLSSHNNHMAKVLTPELYAELRAKCTPSGFTLDDAIQTGVDNPGHPYIM
  TVGAVAGDEESYDVFKDLFDPIIEERHGGYQPSDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGF
  CLPPHCSRGERRAIEKLAVEALSSLDGDLSGRYYALKSMTEAEQQQLIDDHFLFDKPVSPLLLASGMARD
  WPDARGIWHNDNKTFLVWINEEDHLRVISMQKGGNMKEVFTRFCTGLTQIETLFKSKNYEFMWNPHLGYI
  LTCPSNLGTGLRAGVHIKLPHLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEVELV
  QMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021273
• ORF Size: 1143 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021273.3, NP_067248.1
• RefSeq Size: 1478
• RefSeq ORF: 1146
• Locus ID: 12709
• Gene Summary: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. [UniProtKB/Swiss-Prot Function]