Sphk1 (BC037710) Mouse Tagged ORF Clone

CAT#: MG205960

Sphk1 (GFP-tagged) - Mouse sphingosine kinase 1 (cDNA clone MGC:47288 IMAGE:4221357)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sphk1
• Synonyms: SK1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205960 representing BC037710
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCAGTAGAATGCCCTCGAGGACTGCTCCCACGGCCATGCAGAGTGCTGGTGCTGCTGAACCCCC
  AGGGTGGCAAGGGCAAGGCTCTGCAGCTCTTCCAGAGCCGTGTGCAGCCCTTCCTGGAGGAGGCAGAGAT
  AACCTTTAAACTGATACTCACCGAACGGAAGAACCATGCCAGGGAGCTGGTGTGTGCAGAGGAGTTGGGT
  CACTGGGACGCCCTGGCAGTCATGTCCGGTGATGGTCTGATGCATGAGGTGGTGAATGGGCTAATGGAAC
  GGCCCGACTGGGAGACTGCCATCCAGAAACCCCTGTGTAGCCTCCCTGGAGGCTCCGGCAATGCGCTGGC
  AGCTTCTGTGAACCACTATGCTGGGTACGAGCAGGTGACTAATGAAGACCTGCTCATCAACTGCACACTG
  CTGTTGTGCCGCCGGCGCCTGTCACCCATGAACCTGCTGTCCCTGCACACTGCTTCTGGGCTGCGGCTCT
  ATTCTGTGCTCAGTCTGTCCTGGGGCTTTGTTGCTGACGTGGACCTCGAGAGTGAGAAGTACAGGCGCTT
  GGGGGAGATTCGTTTCACAGTGGGCACCTTCTTTCGCCTAGCAAGCCTGCGCATCTACCAAGGCCAACTG
  GCCTACCTTCCTGTAGGAACTGTGGCCTCTAAGAGACCCGCCTCTACACTGGTGCAGAAGGGCCCCGTCG
  ACACACACCTTGTTCCTCTGGAGGAGCCAGTGCCTTCTCATTGGACTGTGGTACCAGAACAGGACTTTGT
  CCTGGTGCTGGTGCTGCTACACACCCACCTGAGCTCCGAGCTGTTTGCAGCACCCATGGGCCGCTGTGAG
  GCTGGTGTTATGCATCTGTTCTACGTACGTGCGGGGGTGTCAAGGGCTGCGCTGCTGCGCCTCTTCCTGG
  CCATGCAGAAGGGCAAGCATATGGAACTTGACTGTCCATACCTGGTTCATGTGCCCGTGGTTGCTTTCCG
  CCTGGAGCCCAGGAGCCAGAGGGGCGTGTTTTCTGTGGATGGAGAGCTGATGGTATGTGAAGCTGTGCAG
  GGCCAAGTGCACCCAAACTACCTTTGGATGGTCTGTGGCAGCAGAGATGCCCCATCCGGCCGGGACTCCC
  GGCGGGGGCCACCTCCAGAAGAACCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC037710
• ORF Size: 1146 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC037710, AAH37710
• RefSeq Size: 1687
• RefSeq ORF: 1148
• Locus ID: 20698
• Gene Summary: This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]