Ptges2 (NM_133783) Mouse Tagged ORF Clone

CAT#: MG205997

Ptges2 (GFP-tagged) - Mouse prostaglandin E synthase 2 (Ptges2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ptges2
• Synonyms: 0610038H10Rik; C79137; Gbf1; Mpges2; Pges2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205997 representing NM_133783
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGGCGGCGCGCCTTTCGTGGGTGCTTGTCTCTAGCCGGTGCGCCCTGACTGAGGGGCTGCTCA
  CACGACCCTGGCAGCCGCTCTCGGCACAGAGCCGGGCTGGCTTCACCAGGGTGGCTGCCGGAAGCCGGGG
  CGCCGCGGTCCGCAAGGGGAGCCCGCGGTTGCTGGGGGCGGCGGCGCTGGCCCTGGGCGGCGCGCTGGGG
  CTGTACCACACCGTGCGGTGGCACCAGCGTTCCCAGGACCTCCGCGCGGAGCGCTCGGCTGCGCAGCTGC
  CCCTGTCGAACAGCCTGCAGCTGACCCTGTACCAGTACAAGACATGTCCCTTCTGCAGCAAAGTCCGTGC
  CTTCCTCGACTTCCACTCCCTGCCCTATCAGGTGGTGGAGGTGAATCCCGTGAGAAGGACTGAGATCAAA
  TTCTCCTCCTACAGGAAAGTGCCCATCTTGGTGGCCCAGGAAGGAGACAGCTTGCAACAGCTCAATGACT
  CCTCTGTCATCATTAGTGCCCTCAAGACCTACCTGGTTTCAGGGCAGCCCCTGGAAGAGGTCATCACTTA
  TTACCCACCCATGAAGGCCATGAATGACCAGGGCAAGGAGGTGACCGAGTTTTGCAACAAGTACTGGCTC
  ATGCTGGACGAGAAGGAGGCCCAGCAGATGTATGGCGGGAAGGAAGCCAGGACGGAGGAGATGAAGTGGC
  GGCAGTGGGCGGATGACTGGCTGGTGCATCTCATCTCTCCCAACGTGTACCGAACACCCGCTGAGGCCTT
  GGCTTCCTTCGACTACATTGTCCGTGAGGGCAAGTTTGGGGCTGTGGAGGCTGCCATGGCCAAGTATGTG
  GGCGCGGCTGCCATGTACCTCATCAGCAAGCGCCTCAAAAGCAGGCACCACCTGCAGGATGATGTACGGG
  TAGACCTCTATGAAGCAGCCAACAAGTGGGTGACAGCCGTGGGTAAAGACCGGCCATTCATGGGGGGTCA
  GAAGCCTAACCTTGCTGACCTGGCAGTGTATGGTGTGCTGCGAGTGATGGAGGGTCTGGAGGCCTTCGAC
  GACCTGATGCGACACTCACACATCCAGCCTTGGTACCTGCGGATGGAGCGGGCCATTGAAGAAGCCCCCT
  CGGTGCATCATGTCAACCCTAGCTGCAAAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205997 representing NM_133783
  Red=Cloning site Green=Tags(s)
  
  MAQAARLSWVLVSSRCALTEGLLTRPWQPLSAQSRAGFTRVAAGSRGAAVRKGSPRLLGAAALALGGALG
  LYHTVRWHQRSQDLRAERSAAQLPLSNSLQLTLYQYKTCPFCSKVRAFLDFHSLPYQVVEVNPVRRTEIK
  FSSYRKVPILVAQEGDSLQQLNDSSVIISALKTYLVSGQPLEEVITYYPPMKAMNDQGKEVTEFCNKYWL
  MLDEKEAQQMYGGKEARTEEMKWRQWADDWLVHLISPNVYRTPAEALASFDYIVREGKFGAVEAAMAKYV
  GAAAMYLISKRLKSRHHLQDDVRVDLYEAANKWVTAVGKDRPFMGGQKPNLADLAVYGVLRVMEGLEAFD
  DLMRHSHIQPWYLRMERAIEEAPSVHHVNPSCKD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133783
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133783.2, NP_598544.2
• RefSeq Size: 1969
• RefSeq ORF: 1155
• Locus ID: 96979
• Gene Summary: The protein encoded by this gene is a Golgi membrane-associated prostaglandin E synthase candidate, which is capable of catalyzing the conversion of prostaglandin H2 to prostaglandin E2 in vitro. However, a study using mice deficient of this gene suggests that this enzyme does not contribute to prostaglandin E2 biosynthesis in vivo. This protein is synthesized as a Golgi membrane-bound protein, but its N-terminal hydrophobic region is cleaved off during protein maturation to produce the predominant soluble truncated form that still retains the enzyme activity. This soluble protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE), possibly via an interaction with CAAAT/enhancer-binding protein-beta. [provided by RefSeq, Oct 2009]