Bcat1 (BC053706) Mouse Tagged ORF Clone

CAT#: MG206032

Bcat1 (GFP-tagged) - Mouse branched chain aminotransferase 1, cytosolic (cDNA clone MGC:60637 IMAGE:30063465)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bcat1
• Synonyms: BCATc
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206032 representing BC053706
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGACTGCAGTAATGGATGCTCCGCGCCGTTTGCTGGAGAAAGAGGATCAGAAGAAGTGGCGGAGA
  CTTTTAGGGCCAAAGATCTCATCATCACACCAGCCACTGTCTTAAAAGAGAAGCCGGACCCGGATTCGCT
  GGTCTTTGGAGCTACGTTTACTGACCACATGCTGACGGTGGAGTGGTCCTCTGCGTCTGGATGGGAGAAA
  CCTCACATTAAGCCTTTTGGAAACTTGCCCATACATCCCGCTGCCTCTGTTTTGCACTACGCTGTGGAAC
  TGTTTGAAGGCTTGAAAGCCTTTCGGGGAGTTGATAACAAGATCCGATTGTTCCGGCCGGACCTCAACAT
  GGATAGAATGTGCCGATCTGCTGTGAGGACCACGCTGCCGATGTTTGACAAGGAGGAGCTCCTAAAGTGT
  ATTCTTCAGCTTCTACAGATCGACCAAGAATGGGTTCCCTACTCCACCTCTGCCAGCCTCTACATCCGCC
  CCACATTTATCGGAACTGAGCCATCTCTTGGCGTCAAGAAGCCTTCCAAAGCCCTACTCTTTGTGATCCT
  GAGCCCCGTGGGACCTTATTTTTCTAGTGGATCTTTTACTCCGGTGTCCCTGTGGGCCAATCCAAAGTAT
  ATCAGAGCCTGGAAAGGTGGGACTGGAGACTGCAAGATGGGCGGCAATTATGGAGCCTCCCTTCTGGCAC
  AGTGCGAGGCCGCGGAGAATGGCTGTCAGCAGGTCCTGTGGCTGTACGGCAAGGACAACCAGATAACTGA
  AGTAGGCACAATGAATCTTTTCCCCTACTGGATAAACGAAGACGGAGAAGAGGAGCTGGCAACGCCCCCA
  CTAGATGGCATCATTCTCCCAGGAGTGACCAGGCAGAGCATCCTGGAGCTGGCACAACAGTGGGGTGAGT
  TTAAGGTATGTGAGAGACACCTCACCATGGATGACCTGGCCACCGCCCTTGAGGGGAACAGAGTGAAGGA
  GATGTTCGGCTCAGGGACAGCCTGCGTTGTCTGCCCAGTCTCTGATATTCTGTACAAGGGCCAGATGCTG
  CATATTCCAACGATGGAGAATGGCCCCAAGCTTGCAAGTCGAATCCTGGGAAAGCTGACTGATATCCAGT
  ATGGAAGGGTGGAGAGTGACTGGACAATCGAGCTACCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206032 representing BC053706
  Red=Cloning site Green=Tags(s)
  
  MKDCSNGCSAPFAGERGSEEVAETFRAKDLIITPATVLKEKPDPDSLVFGATFTDHMLTVEWSSASGWEK
  PHIKPFGNLPIHPAASVLHYAVELFEGLKAFRGVDNKIRLFRPDLNMDRMCRSAVRTTLPMFDKEELLKC
  ILQLLQIDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPSKALLFVILSPVGPYFSSGSFTPVSLWANPKY
  IRAWKGGTGDCKMGGNYGASLLAQCEAAENGCQQVLWLYGKDNQITEVGTMNLFPYWINEDGEEELATPP
  LDGIILPGVTRQSILELAQQWGEFKVCERHLTMDDLATALEGNRVKEMFGSGTACVVCPVSDILYKGQML
  HIPTMENGPKLASRILGKLTDIQYGRVESDWTIELP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC053706
• ORF Size: 1160 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC053706, AAH53706
• RefSeq Size: 2493
• RefSeq ORF: 1160
• Locus ID: 12035
• Gene Summary: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. [UniProtKB/Swiss-Prot Function]