Pdha1 (BC007142) Mouse Tagged ORF Clone

CAT#: MG206079

Pdha1 (GFP-tagged) - Mouse pyruvate dehydrogenase E1 alpha 1 (cDNA clone MGC:6172 IMAGE:3600605)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pdha1
• Synonyms: Pdha-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206079 representing BC007142
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAGATGCTTGCCGCTGTATCCCGCGTGTTGGCAGGCTCTGCGCAGAAGCCGGCAAGCCGAGTGC
  TGGTTGCTTCCCGTAATTTTGCAAATGATGCTACATTTGAGATTAAGAAATGTGACCTTCATCGGCTAGA
  AGAGGGCCCCCCAGTCACCACAGTGCTCACCAGAGAGGATGGGCTCAAGTACTACAGGATGATGCAGACT
  GTGCGCCGGATGGAGCTAAAGGCGGATCAGCTGTATAAGCAGAAAATCATTCGTGGTTTCTGTCACTTGT
  GTGATGGTCAGGAAGCCTGCTGCGTGGGCCTGGAGGCTGGCATAAACCCTACGGACCACCTCATCACTGC
  CTATCGAGCACATGGCTTCACCTTCACTCGGGGCCTGCCTGTGCGAGCAATTCTTGCAGAGCTAACAGGA
  CGAAGAGGAGGTTGTGCTAAAGGGAAAGGCGGGTCAATGCACATGTACGCCAAGAACTTCTATGGAGGCA
  ACGGCATCGTTGGAGCTCAGGTGCCCCTGGGAGCAGGAATTGCCCTGGCCTGCAAGTACAATGGAAAAGA
  TGAGGTCTGTTTGACATTATACGGCGATGGTGCTGCTAATCAGGGTCAGATCTTTGAAGCTTACAATATG
  GCAGCACTGTGGAAATTACCTTGCATTTTTATCTGTGAGAACAACCGCTATGGCATGGGGACGTCTGTTG
  AGAGAGCAGCAGCCAGCACGGACTACTACAAAAGAGGAGATTTTATTCCTGGACTCAGGGTAGATGGAAT
  GGATATCTTGTGCGTCCGAGAGGCAACAAAGTTTGCGGCTGCCTATTGCAGGTCTGGTAAGGGGCCCATC
  CTGATGGAGCTCCAGACTTACCGCTACCATGGACACAGCATGAGTGACCCTGGAGTAAGCTACCGCACTC
  GAGAAGAAATCCAGGAAGTAAGAAGTAAGAGTGACCCTATTATGCTTCTCAAGGATAGAATGGTGAACAG
  CAATCTTGCAAGTGTTGAAGAATTAAAGGAGATTGATGTGGAAGTGAGGAAAGAAATCGAGGATGCTGCC
  CAGTTTGCCACGGCTGATCCTGAGCCCCCGTTGGAGGAACTAGGCTATCACATCTACAGCAGTGATCCTC
  CCTTTGAAGTGCGTGGTGCCAACCAGTGGATCAAGTTTAAGTCAGTCAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206079 representing BC007142
  Red=Cloning site Green=Tags(s)
  
  MRKMLAAVSRVLAGSAQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQT
  VRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLPVRAILAELTG
  RRGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNM
  AALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATKFAAAYCRSGKGPI
  LMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAA
  QFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC007142
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC007142, AAH07142
• RefSeq Size: 2778
• RefSeq ORF: 1172
• Locus ID: 18597