Nme7 (BC049225) Mouse Tagged ORF Clone

CAT#: MG206172

Nme7 (GFP-tagged) - Mouse non-metastatic cells 7, protein expressed in (cDNA clone MGC:54842 IMAGE:3483725)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nme7
• Synonyms: Nm23-M7, Nm23-r7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206172 representing BC049225
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGCCTGTCAGCAGGGAAGAAGTTCCAGTTTGGTTTCTCCATATATGGCACCCAAGAATCAGAGCG
  AGAGATTCGCTTTCATTGCAGAGTGGTATGATCCAAATGCTTCATTGCTCCGACGCTATGAGCTGCTGTT
  TTACCCCACAGACGGATCTGTTGAAATGCATGATGTAAAGAATCGTCGCACCTTCTTAAAGCGGACCAAG
  TATGAGGACCTGCGCCTGGAAGATCTATTTATAGGCAACAAAGTCAATGTGTTTTCTCGACAGCTGGTGT
  TGATTGACTATGGGGACCAATACACAGCCCGCCAGCTGGGCAGCAGGAAAGAGAAAACTTTAGCCCTGAT
  CAAACCAGATGCAGTGTCAAAGGCCGGAGAAATCATTGAGATGATAAACAAAAGTGGATTTACTATAACC
  AAACTCCGAATGATGACTCTGACAAGGAAAGAAGCAGCGGACTTTCATGTAGACCATCACTCAAGGCCTT
  TTTATAACGAACTGATCCAGTTTATCACAAGTGGGCCTGTTATTGCCATGGAGATCTTAAGAGATGACGC
  GATCTGTGAGTGGAAAAGGTTGCTTGGACCCGCAAACTCTGGGCTATCACGGACAGATGCCCCCGGAAGC
  ATCCGAGCCCTCTTTGGGACAGATGGCGTGAGAAATGCAGCTCACAGCCCTGATACTTTTGCATCTGCTG
  CCAGAGAAATGGAATTGTTTTTTCCTTCAAGTGGAGGCTGTGGGCCAGCGAACACTGCTAAATTTACCAA
  TTGCACCTGTTGCATCATTAAGCCTCATGCTATCAGTGAAGGAATGTTGGGAAAGATTTTAATAGCTATT
  CGGGATGCATGCTTTGGAATGTCAGCGATACAGATGTTCAATTTGGATCGGGTTAATGTTGAAGAATTCT
  ATGAAGTCTATAAAGGTGTAGTGTCTGAGTATAATGATATGGTGACAGAGCTGTGCTCCGGCCCTTGCGT
  AGCAATAGAGATCCAACAGAGCAACCCTACAAAGACATTTCGAGAATTCTGCGGACCTGCTGATCCTGAA
  ATCGCCCGGCATTTACGACCTGAGACCCTCAGGGCAATTTTTGGTAAAACTAAGGTTCAAAATGCTGTTC
  ATTGCACGGATCTGCCGGAGGATGGGCTCCTGGAGGTCCAGTATTTCTTCAAGATCTTGGATAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206172 representing BC049225
  Red=Cloning site Green=Tags(s)
  
  MRACQQGRSSSLVSPYMAPKNQSERFAFIAEWYDPNASLLRRYELLFYPTDGSVEMHDVKNRRTFLKRTK
  YEDLRLEDLFIGNKVNVFSRQLVLIDYGDQYTARQLGSRKEKTLALIKPDAVSKAGEIIEMINKSGFTIT
  KLRMMTLTRKEAADFHVDHHSRPFYNELIQFITSGPVIAMEILRDDAICEWKRLLGPANSGLSRTDAPGS
  IRALFGTDGVRNAAHSPDTFASAAREMELFFPSSGGCGPANTAKFTNCTCCIIKPHAISEGMLGKILIAI
  RDACFGMSAIQMFNLDRVNVEEFYEVYKGVVSEYNDMVTELCSGPCVAIEIQQSNPTKTFREFCGPADPE
  IARHLRPETLRAIFGKTKVQNAVHCTDLPEDGLLEVQYFFKILDN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC049225
• ORF Size: 1187 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC049225, AAH49225
• RefSeq Size: 1682
• RefSeq ORF: 1187
• Locus ID: 171567